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| rdf:type
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| Description
| - The amino acid sequence of methylamine oxidase (MeAO) from the fungus Aspergillus niger was analyzed using mass spectrometry (MS). First, MeAO was characterized by an accurate molecular mass of 72.4 kDa of the monomer measured using MALDI-TOF-MS and by a pI value of 5.8 determined by isoelectric focusing. MALDI-TOF-MS revealed a clear peptide mass fingerprint after tryptic digestion, which did not provide any relevant hit when searched against a nonredundant protein database and was different from that of A. niger amine oxidase AO-I. Tandem mass spectrometry with electrospray ionization coupled to liquid chromatography allowed unambiguous reading of six peptide sequences (11-19 amino acids) and seven sequence tags (4-15 amino acids), which were used for MS BLAST homology searching. MeAO was found to be largely homologous to a hypothetical protein AN7641.2 (EMBL/GenBank protein accession code EAA61827) from Aspergillus nidulans FGSC A4 with a theoretical molecular mass of 76 460 Da and pI 6.14, which b
- The amino acid sequence of methylamine oxidase (MeAO) from the fungus Aspergillus niger was analyzed using mass spectrometry (MS). First, MeAO was characterized by an accurate molecular mass of 72.4 kDa of the monomer measured using MALDI-TOF-MS and by a pI value of 5.8 determined by isoelectric focusing. MALDI-TOF-MS revealed a clear peptide mass fingerprint after tryptic digestion, which did not provide any relevant hit when searched against a nonredundant protein database and was different from that of A. niger amine oxidase AO-I. Tandem mass spectrometry with electrospray ionization coupled to liquid chromatography allowed unambiguous reading of six peptide sequences (11-19 amino acids) and seven sequence tags (4-15 amino acids), which were used for MS BLAST homology searching. MeAO was found to be largely homologous to a hypothetical protein AN7641.2 (EMBL/GenBank protein accession code EAA61827) from Aspergillus nidulans FGSC A4 with a theoretical molecular mass of 76 460 Da and pI 6.14, which b (en)
- Aminokyselinová sekvence methylaminoxidasy izolované z plísně Aspergillus niger byla analyzována různými MS technikami. MALDI-MS umožnila stanovení přesné hmoty monomeru o velikosti 72,4 kDa. Isoelektrický bod byl určen pomocí isoelektrické fokusace. Peptidové mapování pomocí MALDI-MS ukázalo, že sekvence methylaminoxidasy se výrazně liší od sekvence aminoxidasy AO-I. Částečná sekvence vyizolované methylaminoxidasy byla určena pomocí konbinace přístupu MALDI-PSD-MS a nanoLC-ESI-MSMS a s využitím MS BLAST softwaru pro hledání homologie podle zadaných peptidových sekvencí proteinu. V databázi byl nalezen homologní hypotetický protein AN7641.2 (EMBL/GenBank protein-accession code EAA61827) z Aspergillus nidulans FGSC A4 o teoretické molekulové hmotnosti 76.46 kDa a pI 6.14, ktery patří do skupiny aminoxidas obsahujících měď. Tento protein vykazuje pouze malou homologii k aminooxidase AO-I (32 % identity, 49 % podobnost). (cs)
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| Title
| - Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger
- Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger (en)
- Mapování primární struktury methylaminoxidasy z Aspergillus niger obsahující topachinon a měď (cs)
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| skos:prefLabel
| - Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger
- Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger (en)
- Mapování primární struktury methylaminoxidasy z Aspergillus niger obsahující topachinon a měď (cs)
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| skos:notation
| - RIV/61989592:15310/05:00002191!RIV06-MSM-15310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ME 664), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/05:00002191
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Aspergillus niger; mass specrometry; methylamine oxidase; peptide; primary structure (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Šebela, Marek
- Frébort, Ivo
- Lenobel, René
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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