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Description
  • Práce se zabývá automatizovaným a manuálním luminescenčním stanovením antioxidační kapacity. Jsou srovnány analytické rysy. (cs)
  • The analytical performances of a manual and a partially automated chemiluminescent (CL) assay, of total antioxidant capacity (TAC) were assessed. In both cases the light emitting reaction involved luminol, horseradish peroxidase and hydrogen peroxyde, but the emission kinetics and the parameters taken into account to calculate TAC values were completely different. The major characteristics expressing the quality of the two analytical methods, i.e. inaccuracy, repeteability and reproducibility, sensitivity, time required for the analysis and detection limit, were estimated by using standard solutions of Trolox. The reliability of the automated method, in comparison with the more validated manual one, was demonstrated testing food samples such as honey, wine and dietary supplements and performing a statistical analysis of the results. The comparison of the two series of data by t-test resulted in p values in the range 0.1-0.01. The time required for the analysis of each sample was reduced to one third u
  • The analytical performances of a manual and a partially automated chemiluminescent (CL) assay, of total antioxidant capacity (TAC) were assessed. In both cases the light emitting reaction involved luminol, horseradish peroxidase and hydrogen peroxyde, but the emission kinetics and the parameters taken into account to calculate TAC values were completely different. The major characteristics expressing the quality of the two analytical methods, i.e. inaccuracy, repeteability and reproducibility, sensitivity, time required for the analysis and detection limit, were estimated by using standard solutions of Trolox. The reliability of the automated method, in comparison with the more validated manual one, was demonstrated testing food samples such as honey, wine and dietary supplements and performing a statistical analysis of the results. The comparison of the two series of data by t-test resulted in p values in the range 0.1-0.01. The time required for the analysis of each sample was reduced to one third u (en)
Title
  • Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison
  • Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison (en)
  • Automatizované a manuální luminescenční stanovení antioxidační kapacity: srovnání analytických rysů (cs)
skos:prefLabel
  • Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison
  • Automated and manual luminescent assay of antioxidant capacity: analytical features by comparison (en)
  • Automatizované a manuální luminescenční stanovení antioxidační kapacity: srovnání analytických rysů (cs)
skos:notation
  • RIV/61989592:15310/04:00002115!RIV/2005/MSM/153105/N
http://linked.open.../vavai/riv/strany
  • 665-670
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 153100007)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 555644
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/04:00002115
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • luminescence;antioxidant capacity;microplate luminometer;foods (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [45D620CE7874]
http://linked.open...i/riv/nazevZdroje
  • Talanta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 64
http://linked.open...iv/tvurceVysledku
  • Sichertová, Dana
  • Fini, F.
  • Girotti, S.
  • Bolelli, L.
  • Budini, R.
  • Ferri, E.
  • Sabatini, A. G.
http://linked.open...n/vavai/riv/zamer
issn
  • 0039-9140
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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