About: Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. (en)
Title	Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters (en)
skos:prefLabel	Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters (en)
skos:notation	RIV/61389030:_____/14:00430550!RIV15-GA0-61389030
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP305/11/0797)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Dobrev, Petre Zažímalová, Eva Petrášek, Jan Skůpa, Petr Simon, Sibu
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	3224
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/14:00430550
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Auxin; Auxin metabolism; BY-2 tobacco cells (en)
http://linked.open.../riv/klicoveSlovo	Auxin Auxin metabolism BY-2 tobacco cells
http://linked.open...ontrolniKodProRIV	[14E4CA2D26B3]
http://linked.open...i/riv/mistoVydani	Totowa
http://linked.open...vEdiceCisloSvazku	Methods in Molecular Biology
http://linked.open...i/riv/nazevZdroje	PLANT CHEMICAL GENOMICS: METHODS AND PROTOCOLS
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...v/pocetStranKnihy	269 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular mechanisms controlling homeostasis of plant growth regulatory compound auxin
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Dobrev, Petre Friml, J. Petrášek, Jan Zažímalová, Eva Skůpa, Petr Simon, Sibu
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-592-7_23
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-62703-591-0

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