About: Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The anatomical characteristics and PA content during cryopreservation and regrowth were studied in the ESMs of AFO 541 and C110 cultures, which have comparable ESM anatomy but diverse growth rates, PA content and regeneration abilities after cryopreservation. The endogenous free PAs were maintained at high levels, with spermidine being the predominant PA in the AFO 541, while in C110 the content of putrescine and spermidine was almost identical and rather low, the content of spermidine being approximately one-third that in the ESM of AFO 541. Osmotic pretreatment, using a double application of sorbitol followed by an application of dimethyl sulfoxide (DMSO) resulted in the continual disintegration of polyembryogenic centers and suspensors in both cell lines. A continual decrease in the level of PAs was observed during the cell osmotic pretreatment. The cells that retained their viability and regrowth ability after cryopreservation were the meristematic cells inside embryonal heads. The anatomical characteristics and PA content during cryopreservation and regrowth were studied in the ESMs of AFO 541 and C110 cultures, which have comparable ESM anatomy but diverse growth rates, PA content and regeneration abilities after cryopreservation. The endogenous free PAs were maintained at high levels, with spermidine being the predominant PA in the AFO 541, while in C110 the content of putrescine and spermidine was almost identical and rather low, the content of spermidine being approximately one-third that in the ESM of AFO 541. Osmotic pretreatment, using a double application of sorbitol followed by an application of dimethyl sulfoxide (DMSO) resulted in the continual disintegration of polyembryogenic centers and suspensors in both cell lines. A continual decrease in the level of PAs was observed during the cell osmotic pretreatment. The cells that retained their viability and regrowth ability after cryopreservation were the meristematic cells inside embryonal heads. (en)
Title	Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures (en)
skos:prefLabel	Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures (en)
skos:notation	RIV/61389030:_____/10:00357035!RIV11-MSM-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(OC 158), P(OC08013), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Cvikrová, Milena Martincová, Olga Vágner, Martin Vondráková, Zuzana Eliášová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	252418
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00357035
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cryopreservation; Norway spruce (Picea abies (L ) Karst); somatic embryogenesis (en)
http://linked.open.../riv/klicoveSlovo	cryopreservation somatic embryogenesis Norway spruce (Picea abies (L ) Karst)
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[9A03C095A65C]
http://linked.open...i/riv/nazevZdroje	Tree Physiology
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Effects of drought and heat stress on polyamine metabolism in wild type and transgenic tobacco plants. http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20158
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	30
http://linked.open...iv/tvurceVysledku	Cvikrová, Milena Martincová, Olga Vágner, Martin Eliášová, Kateřina Vondráková, Zuzana
http://linked.open...ain/vavai/riv/wos	000282818100009
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0829-318X
number of pages	14 (xsd:int)

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