About: Isolation of melatonin by immunoaffinity chromatography.

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About: Isolation of melatonin by immunoaffinity chromatography. Goto Sponge Distinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A single-step, highly specific and easy-to-use method was developed for isolation and purification of melatonin from complex biological matrices. Polyclonal antibodies highly specific against melatonin (with cross-reactivities with related compounds below 0.02%, except for 6-hydroxymelatonin) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of immunoaffinity gel. Melatonin recovery by the immunoaffinity method was ~95%, allowing single-step processing of samples prior to electrospray HPLCůMS analysis (with detection limit 10 fmol). The method was successfully used for determining melatonin in human serum and turned out to be better than the non-specific solid-phase extraction published earlier. A single-step, highly specific and easy-to-use method was developed for isolation and purification of melatonin from complex biological matrices. Polyclonal antibodies highly specific against melatonin (with cross-reactivities with related compounds below 0.02%, except for 6-hydroxymelatonin) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of immunoaffinity gel. Melatonin recovery by the immunoaffinity method was ~95%, allowing single-step processing of samples prior to electrospray HPLCůMS analysis (with detection limit 10 fmol). The method was successfully used for determining melatonin in human serum and turned out to be better than the non-specific solid-phase extraction published earlier. (en)
Title	Isolation of melatonin by immunoaffinity chromatography. Isolation of melatonin by immunoaffinity chromatography. (en)
skos:prefLabel	Isolation of melatonin by immunoaffinity chromatography. Isolation of melatonin by immunoaffinity chromatography. (en)
skos:notation	RIV/61389030:_____/02:56023055!RIV/2003/GA0/A56003/N
http://linked.open.../vavai/riv/strany	9;15
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/02/0475), P(OC 844.10), Z(AV0Z5038910), Z(MSM 153100008)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Siglerová, Věra Rolčík, Jakub Strnad, Miroslav Lenobel, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	649797
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/02:56023055
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Melatonin (en)
http://linked.open.../riv/klicoveSlovo	Melatonin
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[84B26B93C32A]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography. B - Biomedical Sciences and Applications
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Purine inhibitors of cyclin-dependent kinases as anticancer and antimitotic compounds http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20844.10
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	25
http://linked.open...iv/tvurceVysledku	Rolčík, Jakub Strnad, Miroslav Lenobel, René Siglerová, Věra
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100008
issn	0378-4347
number of pages	7 (xsd:int)

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