About: A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I

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About: A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The Type I restriction-modification enzyme EcoR124I is an ATP-dependent endonuclease that uses dsDNA translocation to locate and cleave distant non-specific DNA sites. Bioinformatic analysis of the HsdR subunits of EcoR124I and related Type I enzymes showed that in addition to the principal PD-(E/D)xK Motifs, I, II and III, a QxxxY motif is also present that is characteristic of RecB-family nucleases. The QxxxY motif resides immediately C-terminal to Motif III within a region of predicted -helix. Using mutagenesis, we examined the role of the Q and Y residues in DNA binding, translocation and cleavage. Roles for the QxxxY motif in coordinating the catalytic residues or in stabilizing the nuclease domain on the DNA are discussed The Type I restriction-modification enzyme EcoR124I is an ATP-dependent endonuclease that uses dsDNA translocation to locate and cleave distant non-specific DNA sites. Bioinformatic analysis of the HsdR subunits of EcoR124I and related Type I enzymes showed that in addition to the principal PD-(E/D)xK Motifs, I, II and III, a QxxxY motif is also present that is characteristic of RecB-family nucleases. The QxxxY motif resides immediately C-terminal to Motif III within a region of predicted -helix. Using mutagenesis, we examined the role of the Q and Y residues in DNA binding, translocation and cleavage. Roles for the QxxxY motif in coordinating the catalytic residues or in stabilizing the nuclease domain on the DNA are discussed (en) Restrikčně-modifikační enzym Typu I EcoR124I je ATP-dependentní endonukleáza, která používá translokaci dsDNA ke štěpení ve vzdálených nespecifických místech. Bioinformatická analýza podjednotky HsdR EcoR124I a příbuzných enzymů Typu I ukázala, že kromě principiálních PD-(E/D)xK motivů I, II a III, je přítomen také motiv QxxxY charakteristický pro nukleázy skupiny RecB. Motiv QxxxY je umístěn C-terminálně za motivem III v oblasti předpokládaného -helixu. Použitím mutageneze byla zkoumána úloha aa zbytků Q a Y ve vazbě na DNA, v translokaci a štěpení. Je diskutována role motivu QxxxY při koordinaci katalytických zbytků nebo při stabilizaci nukleázové domény na DNA (cs)
Title	A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I (en) Nukleázový motiv RecB-rodiny u restrikční endonukleázy Typu I EcoR124I (cs)
skos:prefLabel	A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I (en) Nukleázový motiv RecB-rodiny u restrikční endonukleázy Typu I EcoR124I (cs)
skos:notation	RIV/61388971:_____/08:00311266!RIV09-AV0-61388971
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/07/0325), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Weiserová, Marie Šišáková, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	354439
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/08:00311266
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	restriction endonuclease; mutagenesis; dsdna (en)
http://linked.open.../riv/klicoveSlovo	mutagenesis dsdna restriction endonuclease
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[79048207AF0C]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Identification of amino acid residues of the HsdS and HsdR subunits required for correct assembly of Type I restriction-modification enzyme EcoR124I
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	36
http://linked.open...iv/tvurceVysledku	Weiserová, Marie Šišáková, Eva Stanley, L. K. Szczelkun, M. D.
http://linked.open...ain/vavai/riv/wos	000257578600007
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0305-1048
number of pages	11 (xsd:int)

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