About: Molecular Evidence of Bartonella DNA in Ixodid Ticks in Czechia

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byla vyvinuta nová metoda detekce DNA rodu Bartonella v klíšťatech. Metoda je založena na amplifikaci části genu pro 16S rRNA a 16S-23S rRNA intergenové oblasti rodu Bartonella pomocí PCR a následnou hybridizací specifickou sondou DNA. Analýza 327 klíštích jedinců z z různých oblastí České Republiky (sběr proveden v letech 2005-2007) prokázal přítomnost DNA rodu Bartonella v 1.2% případů (cs) We report the development of a novel method for detection of Bartonella DNA in ixodid ticks. The assay was based on a specific amplification of a part of 16S rRNA gene and 16S-23S rRNA intergenic spacer region of Bartonella sp. by nested PCR and Southern blot hybridization with specific DNA probe. The method is highly sensitive and specific. The screening of 327 unfed ticks collected in different urban and suburban areas of the Czech Republic from 2003 to 2005 revealed the presence of Bartonella DNA in four Ixodes ricinus individuals (1.2%), two males, one female and one nymph We report the development of a novel method for detection of Bartonella DNA in ixodid ticks. The assay was based on a specific amplification of a part of 16S rRNA gene and 16S-23S rRNA intergenic spacer region of Bartonella sp. by nested PCR and Southern blot hybridization with specific DNA probe. The method is highly sensitive and specific. The screening of 327 unfed ticks collected in different urban and suburban areas of the Czech Republic from 2003 to 2005 revealed the presence of Bartonella DNA in four Ixodes ricinus individuals (1.2%), two males, one female and one nymph (en)
Title	Molecular Evidence of Bartonella DNA in Ixodid Ticks in Czechia Molecular Evidence of Bartonella DNA in Ixodid Ticks in Czechia (en) Molekulární důkaz přítomnosti DNA rodu Bartonella v dospělých klíšťatech v České republice (cs)
skos:prefLabel	Molecular Evidence of Bartonella DNA in Ixodid Ticks in Czechia Molecular Evidence of Bartonella DNA in Ixodid Ticks in Czechia (en) Molekulární důkaz přítomnosti DNA rodu Bartonella v dospělých klíšťatech v České republice (cs)
skos:notation	RIV/61388971:_____/07:00098984!RIV08-AV0-61388971
http://linked.open.../vavai/riv/strany	503;509
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP204/02/D121), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Janeček, Jiří Branny, Pavel Hercík, Kamil
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	434855
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/07:00098984
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bartonella dna; ixodid ticks; hybridization (en)
http://linked.open.../riv/klicoveSlovo	hybridization bartonella dna ixodid ticks
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[44B1E2061F9C]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Two component signal transduction system in Bartonella henselae: structure-function relationships. Epidemiology of the members of the genus Bartonella in ticks
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	52
http://linked.open...iv/tvurceVysledku	Branny, Pavel Hercík, Kamil Janeček, Jiří Hášová, V.
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0015-5632
number of pages	7 (xsd:int)

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