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  • A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers.
  • A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers. (en)
Title
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction (en)
skos:prefLabel
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction
  • Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction (en)
skos:notation
  • RIV/61388963:_____/12:00377953!RIV13-AV0-61388963
http://linked.open...avai/riv/aktivita
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  • I, P(GA203/09/0317)
http://linked.open...iv/cisloPeriodika
  • 55
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  • 161362
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  • RIV/61388963:_____/12:00377953
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  • cross-coupling reactions; nucleoside triphosphates; functionalized DNA; restriction endonucleases; polymerase incorporation (en)
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  • GB - Spojené království Velké Británie a Severního Irska
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  • [8E3AE809B893]
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  • Chemical Communications
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  • 48
http://linked.open...iv/tvurceVysledku
  • Hocek, Michal
  • Ménová, Petra
http://linked.open...ain/vavai/riv/wos
  • 000305255300010
issn
  • 1359-7345
number of pages
http://bibframe.org/vocab/doi
  • 10.1039/c2cc32930a
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