About: Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR). A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR). (en)
Title	Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA (en)
skos:prefLabel	Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA (en)
skos:notation	RIV/61388963:_____/10:00353703!RIV11-AV0-61388963
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z40550506)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Macíčková-Cahová, Hana Hocek, Michal Vrábel, Milan
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav organické chemie a biochemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	252392
http://linked.open...ai/riv/idVysledku	RIV/61388963:_____/10:00353703
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nucleoside triphosphates; DNA polymerase; cross-coupling; DNA conjugates (en)
http://linked.open.../riv/klicoveSlovo	DNA polymerase cross-coupling nucleoside triphosphates DNA conjugates
http://linked.open...ontrolniKodProRIV	[FD829C54B154]
http://linked.open...i/riv/mistoVydani	New York
http://linked.open...i/riv/nazevZdroje	Current Protocols in Chemical Biology
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	550 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Hocek, Michal Macíčková-Cahová, Hana Vrábel, Milan
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství
number of pages	14 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Wiley Interscience
https://schema.org/isbn	9780470559277

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