About: How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy.

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About: How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy. Goto Sponge Distinct Permalink

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Description	Confocal fluorescence correlation spectroscopy (FCS) allows for the determination of lateral diffusion coefficients and surface densities in planar phospholipid systems. The determination of the z-position of the laser focus relative to the phospholipid surface plane is of crucial importance for the accuracy of the confocal FCS experiment. In this work we are determining for the first time this z-position of the laser focus by a so-called 'z-scan', which is based on the determination of diffusion times and particle numbers in 0.1 m steps along the z-axis. Experiments on supported phospholipid bilayers composed of dioleoyl-phosphatidylcholine (DOPC) and small amounts of Rhodamine Red-X 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Rhodamine Red-X DHPE) adsorbed onto atomically flat mica demonstrate that results obtained by the 'z-scan' approach are significantly more precise than those results obtained when the fluorescence. Confocal fluorescence correlation spectroscopy (FCS) allows for the determination of lateral diffusion coefficients and surface densities in planar phospholipid systems. The determination of the z-position of the laser focus relative to the phospholipid surface plane is of crucial importance for the accuracy of the confocal FCS experiment. In this work we are determining for the first time this z-position of the laser focus by a so-called 'z-scan', which is based on the determination of diffusion times and particle numbers in 0.1 m steps along the z-axis. Experiments on supported phospholipid bilayers composed of dioleoyl-phosphatidylcholine (DOPC) and small amounts of Rhodamine Red-X 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Rhodamine Red-X DHPE) adsorbed onto atomically flat mica demonstrate that results obtained by the 'z-scan' approach are significantly more precise than those results obtained when the fluorescence. (en)
Title	How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy. How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy. (en)
skos:prefLabel	How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy. How to Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy. (en)
skos:notation	RIV/61388955:_____/03:54030048!RIV/2004/AV0/A54004/N
http://linked.open.../vavai/riv/strany	4120;4126
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LN00A032), Z(AV0Z4040901)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Lhotský, Alexandr Beneš, Martin Hof, Martin Benda, Aleš Mareček, Vladimír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	609366
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/03:54030048
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence; diffusion; spectroscopy (en)
http://linked.open.../riv/klicoveSlovo	fluorescence spectroscopy diffusion
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F0388443F53B]
http://linked.open...i/riv/nazevZdroje	Langmuir
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure and dynamics of complex molecular systems and biomolecules
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Hof, Martin Beneš, Martin Benda, Aleš Mareček, Vladimír Lhotský, Alexandr Hermens, W. Th.
http://linked.open...n/vavai/riv/zamer	Objasňování zákonitostí vztahů struktury a vlastností - zejména dynamiky a chemické reaktivity - látek a molekulárních systémů
issn	0743-7463
number of pages	7 (xsd:int)

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