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rdf:type
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Description
| - NMR spectra of large proteins are frequently so complicated that even using of triple resonance, multidimensional experiments does not help. In the process of resonance assignment it is inevitable to find responses of amino acid residues with typical cross peak patterns, which then serve as starting points. In case of heavily overlapped resonances, amino acid selective 1H-15N spectra might serve this purpose [1,2]. The selectivity of these experiments is based on a topology of side chains typical for each amino acid residue. The experiments utilize either multiplicity selective in-phase coherence transfer MUSIC (for selection of Gly, Ala, Thr, Ile, Val, Asn, Gln), selective excitation (Ser) or other typical features like e.g. an absence of amino proton(Pro). We have used this strategy to speed up the assignment of 1H, 13C, and 15N resonances of Mason-Pfizer monkey virus protease. A combination of intra- and inter-residual coherence transfer experiments, e.g. Gly(i)-Gly(i+1) or Ala(i)-Ala(i+1) and oth
- NMR spectra of large proteins are frequently so complicated that even using of triple resonance, multidimensional experiments does not help. In the process of resonance assignment it is inevitable to find responses of amino acid residues with typical cross peak patterns, which then serve as starting points. In case of heavily overlapped resonances, amino acid selective 1H-15N spectra might serve this purpose [1,2]. The selectivity of these experiments is based on a topology of side chains typical for each amino acid residue. The experiments utilize either multiplicity selective in-phase coherence transfer MUSIC (for selection of Gly, Ala, Thr, Ile, Val, Asn, Gln), selective excitation (Ser) or other typical features like e.g. an absence of amino proton(Pro). We have used this strategy to speed up the assignment of 1H, 13C, and 15N resonances of Mason-Pfizer monkey virus protease. A combination of intra- and inter-residual coherence transfer experiments, e.g. Gly(i)-Gly(i+1) or Ala(i)-Ala(i+1) and oth (en)
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Title
| - Simplification of NMR spectra of Mason-Pfizer monkey protease.
- Simplification of NMR spectra of Mason-Pfizer monkey protease. (en)
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skos:prefLabel
| - Simplification of NMR spectra of Mason-Pfizer monkey protease.
- Simplification of NMR spectra of Mason-Pfizer monkey protease. (en)
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skos:notation
| - RIV/60461373:22640/01:00004559!RIV/2004/GA0/226404/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22640/01:00004559
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - NMR spectroscopy, protease, Mason-Pfizer virus (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - Central European NMR Discussion Groups
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Hrabal, Richard
- Veverka, Václav
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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