About: Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools     Goto   Sponge   Distinct   Permalink

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Description
  • A combination of direct analysis in real time (DART) ionization coupled to time-of-flight mass spectrometry (TOFMS) and chemometrics was used for animal fat (lard and beef tallow) authentication. This novel instrumentation was employed for rapid profiling of triacylglycerols (TAGs) and polar compounds present in fat samples and their mixtures. Additionally, fat isolated from pork, beef, and pork/beef admixtures was analyzed. Mass spectral records were processed by principal component analysis (PCA) and stepwise linear discriminant analysis (LDA). DART-TOFMS profiles of TAGs were found to be more suitable for the purpose of discrimination among the examined fat types as compared to profiles of polar compounds. The LDA model developed using TAG data enabled not only reliable classification of samples representing neat fats but also detection of admixed lard and tallow at adulteration levels of 5 and 10% (w/w), respectively. The presented approach was also successfully applied to minced meat prepared from pork and beef with comparable fat content. Using the DART-TOFMS TAG profiles of fat isolated from meat mixtures, detection of 10% pork added to beef and vice versa was possible.
  • A combination of direct analysis in real time (DART) ionization coupled to time-of-flight mass spectrometry (TOFMS) and chemometrics was used for animal fat (lard and beef tallow) authentication. This novel instrumentation was employed for rapid profiling of triacylglycerols (TAGs) and polar compounds present in fat samples and their mixtures. Additionally, fat isolated from pork, beef, and pork/beef admixtures was analyzed. Mass spectral records were processed by principal component analysis (PCA) and stepwise linear discriminant analysis (LDA). DART-TOFMS profiles of TAGs were found to be more suitable for the purpose of discrimination among the examined fat types as compared to profiles of polar compounds. The LDA model developed using TAG data enabled not only reliable classification of samples representing neat fats but also detection of admixed lard and tallow at adulteration levels of 5 and 10% (w/w), respectively. The presented approach was also successfully applied to minced meat prepared from pork and beef with comparable fat content. Using the DART-TOFMS TAG profiles of fat isolated from meat mixtures, detection of 10% pork added to beef and vice versa was possible. (en)
Title
  • Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools
  • Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools (en)
skos:prefLabel
  • Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools
  • Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools (en)
skos:notation
  • RIV/60461373:22330/11:43892757!RIV12-MSM-22330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika
  • 11
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 187510
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/11:43892757
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • multivariate analysis; meat; mass spectrometry; direct analysis in real time; Animal fat; Adulteration (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [2D6437AF9270]
http://linked.open...i/riv/nazevZdroje
  • Journal of Agricultural and Food Chemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 59
http://linked.open...iv/tvurceVysledku
  • Hajšlová, Jana
  • Hrbek, Vojtěch
  • Pipek, Petr
  • Rohlík, Bo-Anne
  • Václavík, Lukáš
  • Čajka, Tomáš
http://linked.open...ain/vavai/riv/wos
  • 000291074700018
http://linked.open...n/vavai/riv/zamer
issn
  • 0021-8561
number of pages
http://bibframe.org/vocab/doi
  • 10.1021/jf200734x
http://localhost/t...ganizacniJednotka
  • 22330
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