About: Viability determination by flow cytometry during ABE fermentation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The acetone-butanol-ethanol (ABE) fermentation is a biotechnological process of solvent production by bacteria of the genus Clostridium. The aim of the work was to develop the method for viability determination of Clostridium pasteurianum and apply it for monitoring of ABE fermentation. First, seven fluorescent probes were tested in order to distinguish live and dead clostridial cells in suspension (propidium iodide, sytox green, ethidium bromide, fluorescein diacetate, 5(6)-carboxyfluorescein diacetate, rhodamine 123, bis-((1, 3-dibutylbarburic acid) trimethine) oxonol-BOX). BOX was chosen as the most convenient one for viability determination of C. pasteurianum and the method of staining was subsequently optimised (concentration of BOX: 1 ?g.ml-1, incubation time: 7 min, concentration of cells: OD600nm = 0.20 ? 0.01, demineralised water). Then the fluorescent staining method was applied to batch fermentation process carried out in a laboratory bioreactor under anaerobic conditions. Flow cytometry The acetone-butanol-ethanol (ABE) fermentation is a biotechnological process of solvent production by bacteria of the genus Clostridium. The aim of the work was to develop the method for viability determination of Clostridium pasteurianum and apply it for monitoring of ABE fermentation. First, seven fluorescent probes were tested in order to distinguish live and dead clostridial cells in suspension (propidium iodide, sytox green, ethidium bromide, fluorescein diacetate, 5(6)-carboxyfluorescein diacetate, rhodamine 123, bis-((1, 3-dibutylbarburic acid) trimethine) oxonol-BOX). BOX was chosen as the most convenient one for viability determination of C. pasteurianum and the method of staining was subsequently optimised (concentration of BOX: 1 ?g.ml-1, incubation time: 7 min, concentration of cells: OD600nm = 0.20 ? 0.01, demineralised water). Then the fluorescent staining method was applied to batch fermentation process carried out in a laboratory bioreactor under anaerobic conditions. Flow cytometry (en)
Title	Viability determination by flow cytometry during ABE fermentation Viability determination by flow cytometry during ABE fermentation (en)
skos:prefLabel	Viability determination by flow cytometry during ABE fermentation Viability determination by flow cytometry during ABE fermentation (en)
skos:notation	RIV/60461373:22330/10:00023767!RIV11-MPO-22330___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(FR-TI1/218), P(QH81323), S, Z(MSM6046137305)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Melzoch, Karel Rychtera, Mojmír Patáková, Petra Fribert, Petr Linhová, Michaela Lipovský, Jakub Branská, Barbora
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	295629
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/10:00023767
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Clostridium pasteurianum; flow cytometry; 1-butanol; viability (en)
http://linked.open.../riv/klicoveSlovo	viability 1-butanol Clostridium pasteurianum flow cytometry
http://linked.open...ontrolniKodProRIV	[DF776C86EE53]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	CHISA 2010
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	*THE DESIGN OF THE PRODUCTION OF BUTANOL WITH THE PILOT PLANT TEST VERIFICATION Biobutanol as a perspective renewable source of energy for transport
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Fribert, Petr Linhová, Michaela Lipovský, Jakub Melzoch, Karel Patáková, Petra Rychtera, Mojmír Branská, Barbora
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2010-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
number of pages	12 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Process Engineering Publisher, Novosad, J.
https://schema.org/isbn	978-80-02-02210-7
http://localhost/t...ganizacniJednotka	22330

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