About: Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain

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About: Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two types of lipases (extracellular and cell-bound) were produced by Geotrichum candidum 4013 in liquid medium and were used as biocatalysts in blackcurrant oil hydrolysis. Reaction products were analysed for the degree of conversion from which enzyme activity was evaluated, and the composition of free fatty acids was compared to the composition of oil substrate. The enzyme activity was measured also before and after the reaction in SC-CO2. The fatty acid composition of the acids liberated from oil by hydrolysis suggests a specificity of the cell-bound and extracellular enzymes from Geotrichum candidum 4013. The extracellular lipase displays low selectivity to the polyunsaturated fatty acids, and the cell-bound lipase possesses selectivity to the saturated fatty acids. Enantioselectivity of the tested processes achieved with both induced enzymes was high (from 43 to 242). The activity of all enzymes has markedly increased after their exposure to SC-CO2. The treatment of enzymes by SC-CO2 could be easy Two types of lipases (extracellular and cell-bound) were produced by Geotrichum candidum 4013 in liquid medium and were used as biocatalysts in blackcurrant oil hydrolysis. Reaction products were analysed for the degree of conversion from which enzyme activity was evaluated, and the composition of free fatty acids was compared to the composition of oil substrate. The enzyme activity was measured also before and after the reaction in SC-CO2. The fatty acid composition of the acids liberated from oil by hydrolysis suggests a specificity of the cell-bound and extracellular enzymes from Geotrichum candidum 4013. The extracellular lipase displays low selectivity to the polyunsaturated fatty acids, and the cell-bound lipase possesses selectivity to the saturated fatty acids. Enantioselectivity of the tested processes achieved with both induced enzymes was high (from 43 to 242). The activity of all enzymes has markedly increased after their exposure to SC-CO2. The treatment of enzymes by SC-CO2 could be easy (en)
Title	Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain (en)
skos:prefLabel	Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain (en)
skos:notation	RIV/60461373:22330/09:00022178!RIV10-MSM-22330___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	61
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Macková, Martina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	316378
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/09:00022178
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Geotrichum candidum 4013; lipase; cell-bound (en)
http://linked.open.../riv/klicoveSlovo	Geotrichum candidum 4013 cell-bound lipase
http://linked.open...odStatuVydavatele	BE - Belgické království
http://linked.open...ontrolniKodProRIV	[90679A987A26]
http://linked.open...i/riv/nazevZdroje	Journal of Molecular Catalysis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Macková, Martina Wimmer, Zdeněk Sovová, Helena Hlavsová, Klára Zarevúcká, Marie
http://linked.open...ain/vavai/riv/wos	000272064600013
http://linked.open...n/vavai/riv/zamer	Teoretické základy potravinářských a biochemických technologií
issn	1381-1169
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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