About: Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this paper, we report a large-scale survey for the incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) in stone fruit collections and commercial orchards in the Czech Republic. From the 645 samples analysed, PLMVd was detected in 80 (26.6%) of peaches and the HSVd in 3 (1.3%) of apricot and 1 (0.33%) of peach trees. Sixty-seven accession of peach (44.6%) from the Czech Clonal GeneBank were infected by PLMVd. In addition, we used naturally infected trees to standardise the simultaneous detection of PLMVd and HSVd plus host mRNA as the control by means of one-step multiplex RTC-PCR. Eleven PLMVd and two HSVd isolates were sequenced and analysed. All the PLMVd variants were highly homologous (97–100%) to previously reported PLMVd variants from Tunisian peach and almond trees, and clustered together in the previously reported phylogenetic group III. The HSVd variants obtained from apricot and peach trees were included in the previously proposed recombinant gr In this paper, we report a large-scale survey for the incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) in stone fruit collections and commercial orchards in the Czech Republic. From the 645 samples analysed, PLMVd was detected in 80 (26.6%) of peaches and the HSVd in 3 (1.3%) of apricot and 1 (0.33%) of peach trees. Sixty-seven accession of peach (44.6%) from the Czech Clonal GeneBank were infected by PLMVd. In addition, we used naturally infected trees to standardise the simultaneous detection of PLMVd and HSVd plus host mRNA as the control by means of one-step multiplex RTC-PCR. Eleven PLMVd and two HSVd isolates were sequenced and analysed. All the PLMVd variants were highly homologous (97–100%) to previously reported PLMVd variants from Tunisian peach and almond trees, and clustered together in the previously reported phylogenetic group III. The HSVd variants obtained from apricot and peach trees were included in the previously proposed recombinant gr (en)
Title	Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic (en)
skos:prefLabel	Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic Simultaneous detection and genetic variability of stone fruit viroids in the Czech Republic (en)
skos:notation	RIV/60460709:41210/09:31358!RIV10-MZE-41210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QG60123)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Ryšánek, Pavel Hassan, Mohamed
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	341399
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/09:31358
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Prunus . PLMVd . HSVd . Multiplex RT-PCR; Stone fruit viroids (en)
http://linked.open.../riv/klicoveSlovo	Prunus . PLMVd . HSVd . Multiplex RT-PCR Stone fruit viroids
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[EBE84269DAE0]
http://linked.open...i/riv/nazevZdroje	European Journal of Plant Pathology
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and innovation of diagnostic procedures for economically significant, controlled and quarantine plant pathogenic organisms for the certification program of fruit trees with emphasis on molecular methods
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	124
http://linked.open...iv/tvurceVysledku	Ryšánek, Pavel Hassan, Mohamed Goméz, G. Myrta, Arben Pallás, V.
issn	0929-1873
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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