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| Description
| - Ditylenchus dipsaci, the stem nematode , is a migratory endoparasite of over 500 species of angiosperms. Biological responses and developmental processes are precisely controlled at the level of gene expression. Most pathogenicity factors are not expresse consitutively, but in highly coordinated way. A relatively simple method for profiling differential gene expression is cDNA-amplified fragment lenght polymorphism (cDNA-AFLP). We aimed to used the cDNA-AFLP technique to sample the D. dipsaci transcriptome and discovere genes specifically up-regulated during host infection. A random sample of six TDFs representating gene transcripts more abundant or specifically PCR amplified from infested plant tissues cDNAs, but not from non-infested plant material and nematode suspension cDNAs were cloned and sequenced after PCR re-amplification. Each sequence was compared by the BLASTX algorithm to sequences within the GenBank database. Besides similarities with Caenorhabditis elegans proteins, one TDF was signi
- Ditylenchus dipsaci, the stem nematode , is a migratory endoparasite of over 500 species of angiosperms. Biological responses and developmental processes are precisely controlled at the level of gene expression. Most pathogenicity factors are not expresse consitutively, but in highly coordinated way. A relatively simple method for profiling differential gene expression is cDNA-amplified fragment lenght polymorphism (cDNA-AFLP). We aimed to used the cDNA-AFLP technique to sample the D. dipsaci transcriptome and discovere genes specifically up-regulated during host infection. A random sample of six TDFs representating gene transcripts more abundant or specifically PCR amplified from infested plant tissues cDNAs, but not from non-infested plant material and nematode suspension cDNAs were cloned and sequenced after PCR re-amplification. Each sequence was compared by the BLASTX algorithm to sequences within the GenBank database. Besides similarities with Caenorhabditis elegans proteins, one TDF was signi (en)
- Ditylenchus dipsaci je volně žijící osní fytoparasitický nematod, který se vyskytuje v mnoha biologických rasách. Identifikace a funkční analýza faktorů pathogenicity produkovaných fytoparasitickými nematody je nezbytným předpokladem efektivního šlechtění a transgenozy rostlin .Jednotlivé vývojové procesy v průběhu ontogenese jsou na molekulární úrovni určeny změnami v genové expresi. Jedním z efektivních přístupů ke studiu koordinovaných transkriptomů patogen-rostlina je strategie RNA „fingerprintingu“ označovaná jako cDNA-AFLP. V naší práci jsme použili tuto strategii pro charakterizaci transkriptomu D. dipsaci v průběhu infekčního procesu. Náhodně bylo vybráno šest diferenciálně exprimovaných fragmentů, které byly následně klonovány a sekvenovány. Získané nukleotidové sekvence byly porovnány se sekvencemi v databázi GenBank pomocí modulu BLASTX. Kromě podobnosti s proteinovými sekvencemi Caenorhabditis elegans, jedna sekvence vykazuje vysokou míru podobnosti s chitinasou Heterodera glycine. Na zák (cs)
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| Title
| - An Efficent CDNA-AFLP – Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci,
- An Efficent CDNA-AFLP – Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci, (en)
- Identifikace faktorů pathogenicity fytoparasitických nematod Ditylenchus dipsaci pomocí cDNA-AFLP strategie (cs)
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| skos:prefLabel
| - An Efficent CDNA-AFLP – Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci,
- An Efficent CDNA-AFLP – Based Strategy for the Identification of Pathogenity Factors from the stem Nematode Ditylenchus dipsaci, (en)
- Identifikace faktorů pathogenicity fytoparasitických nematod Ditylenchus dipsaci pomocí cDNA-AFLP strategie (cs)
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| skos:notation
| - RIV/60460709:41210/05:11446!RIV06-MSM-41210___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/05:11446
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - stem nematode, Ditylenchus dipsaci, differential gene expression, TDF-transcript-derived fragment, cDNA-AFLP, bioinformatic analyses, pathogenicity factors. (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...vEdiceCisloSvazku
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| http://linked.open...i/riv/nazevZdroje
| - Sborník abstraktů z konference posluchačů postgraduálního doktorského studia MendelNet 05 Agro, Mendelova zemědělská a lesnická univerzita v Brně
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Marek, Martin
- Ryšánek, Pavel
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Mendelova zemědělská a lesnická univerzita v Brně
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
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