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rdf:type
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Description
| - A set of dominant and co-dominant PCR markers was used to detect the Vf alleles. This methodological procedure was verified on a collection of apple tree varieties with a declared resistance against scab, determined by the dominant Vf gene. Nine reference varieties containing the Vf gene were used, developed by the Research and Breeding Institute of Pomology in Holovousy. Conducted was also a segregation analysis of seedlings of eight complex combination crossbreeds. The resistance or sensitivity of theassessed plant material was also classified in standard greenhouse infection tests. The so-called multi-PCR was used to detect alleles of the Vf gene. The actual amplification and sequences of primer pairs were obtained from Tartarini et al. (1999) exp eriments. The multi-PCR used allowed a simultaneous amplification of one dominant and one co-dominant marker. The co-dominant marker enabled the dominant homozygotes, heterozygotes and recessive homozygotes to be distinguished from each other. The domina
- A set of dominant and co-dominant PCR markers was used to detect the Vf alleles. This methodological procedure was verified on a collection of apple tree varieties with a declared resistance against scab, determined by the dominant Vf gene. Nine reference varieties containing the Vf gene were used, developed by the Research and Breeding Institute of Pomology in Holovousy. Conducted was also a segregation analysis of seedlings of eight complex combination crossbreeds. The resistance or sensitivity of theassessed plant material was also classified in standard greenhouse infection tests. The so-called multi-PCR was used to detect alleles of the Vf gene. The actual amplification and sequences of primer pairs were obtained from Tartarini et al. (1999) exp eriments. The multi-PCR used allowed a simultaneous amplification of one dominant and one co-dominant marker. The co-dominant marker enabled the dominant homozygotes, heterozygotes and recessive homozygotes to be distinguished from each other. The domina (en)
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Title
| - Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.)
- Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.) (en)
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skos:prefLabel
| - Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.)
- Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.) (en)
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skos:notation
| - RIV/60460709:41210/01:00002909!RIV/2002/MSM/412102/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/01:00002909
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Apple, (Malus sp.), scab, (Venturia inaequalis CKE.), DNA, molecular markers, multi PCR, Vf gene (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - 4th International Symposium in the Series Recent Advances in Plant Biotechnology, Plant Molecular Biology for the New Millennium, Book of Abstracts
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Vejl, Pavel
- Blažek, Pavel
- Drahošová, H.
- Skupinová, Sylva
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Ústav molekulární biologie rostlin AV ČR
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http://localhost/t...ganizacniJednotka
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