About: Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid–liquid extraction of the resulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column. Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid–liquid extraction of the resulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column. (en)
Title	Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography (en)
skos:prefLabel	Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography (en)
skos:notation	RIV/60077344:_____/12:00369909!RIV12-MSM-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(7F09028), P(GA203/09/2014), P(NS9755), Z(AV0Z50070508)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Šimek, Petr Zahradníčková, Helena Hušek, Petr
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	138824
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/12:00369909
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	amino acid; serum; plasma (en)
http://linked.open.../riv/klicoveSlovo	plasma amino acid serum
http://linked.open...ontrolniKodProRIV	[FD248E344343]
http://linked.open...i/riv/mistoVydani	New York
http://linked.open...i/riv/nazevZdroje	Amino Acid Analysis. Methods and Protocols
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	363 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Quantitative profiling of metabolites involved in one-carbon metabolism for large scale clinical and experimental studies from birth to adolescence New approaches in body fluid metabolic profiling and their usage in clinical diagnostics. New approaches to alkylchloroformate-mediated sample preparation for parallel GC-MS and LC-MS for metabolite analysis in biofluids
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Hušek, Petr Šimek, Petr Zahradníčková, Helena
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-61779-445-2_13
http://purl.org/ne...btex#hasPublisher	Springer Science & Business Media
https://schema.org/isbn	978-1-61779-444-5

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