About: RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	RNA interference (RNAi) is a technique to selectively suppress mRNA of individual genes and, consequently, their cognate proteins. In consideration of large-scale screens to identify candidate drug targets, we examined the selectivity and sensitivity (the degree of suppression) of RNAi for 11 genes produced in different tissues of the parasite: the gut, tegument (surface) and otherwise. We used the schistosomulum stage prepared from infective cercariae larvae which are accessible in large numbers and adaptable to automated screening platforms. We found that RNAi suppresses transcripts selectively, however, the sensitivity of suppression varies. Additionally, we defined operational parameters to facilitate large-scale RNAi, including choice of culture medium, transfection strategy to deliver dsRNA, dose- and time-dependency, and dosing limits. Finally, using fluorescent probes, we show that the developing gut allows rapid entrance of dsRNA into the parasite to initiate RNAi. RNA interference (RNAi) is a technique to selectively suppress mRNA of individual genes and, consequently, their cognate proteins. In consideration of large-scale screens to identify candidate drug targets, we examined the selectivity and sensitivity (the degree of suppression) of RNAi for 11 genes produced in different tissues of the parasite: the gut, tegument (surface) and otherwise. We used the schistosomulum stage prepared from infective cercariae larvae which are accessible in large numbers and adaptable to automated screening platforms. We found that RNAi suppresses transcripts selectively, however, the sensitivity of suppression varies. Additionally, we defined operational parameters to facilitate large-scale RNAi, including choice of culture medium, transfection strategy to deliver dsRNA, dose- and time-dependency, and dosing limits. Finally, using fluorescent probes, we show that the developing gut allows rapid entrance of dsRNA into the parasite to initiate RNAi. (en)
Title	RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening (en)
skos:prefLabel	RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening (en)
skos:notation	RIV/60077344:_____/10:00349491!RIV12-MSM-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/09/1585), P(KJB600960911), P(LC06009), P(ME10011), Z(AV0Z40550506), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Dvořák, Jan Sojka, Daniel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	285527
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/10:00349491
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Tropical disease; Schistosoma; schistosomula; RNAi; protease; tegument; drug target; drug discovery (en)
http://linked.open.../riv/klicoveSlovo	RNAi drug target drug discovery protease Tropical disease schistosomula tegument Schistosoma
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2B51C93FFAB4]
http://linked.open...i/riv/nazevZdroje	PLoS Neglected Tropical Diseases
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for molecular ecology of vectors and pathogens Parasite-targeted peptidase inhibitors as a tool for schistosomiasis therapy. Hemoglobinolytic aspartic peptidase IrCD - a member of the gut-associated digestive enzyme complex in the tick I. ricinus Trypsin-like proteases from human parasite, blood fluke Schistosoma mansoni
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Dvořák, Jan Horn, Martin Sojka, Daniel Caffrey, C. R. McKerrow, J. H. Lim, K.-C. Braschi, S. Hopkins, S. D. Ruelas, D. Suzuki, B. Štefanič, S.
http://linked.open...ain/vavai/riv/wos	000283559600019
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	1935-2735
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pntd.0000850

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