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rdf:type
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Description
| - Fluorogenní substrát ELF97 fosfát je hydrolyzován enzymem fosfatázou přičemž vzniká fluorescenční nerozpustný produkt ELF 97 alkohol (ELFA). Ve fosforem limitovaných bakteriálních kulturách bylo vysoké procento buněk značeno ELFA (26–100% v závislosti na kmenu). Naproti tomu ve vzorcích ze Středozemního moře to bylo jen 0,01%, přesto že byla prokázána limitace fosforem. (cs)
- The fluorogenic substrate Enzyme-Labeled Fluorescence 97 (ELF-P) is hydrolyzed by the P-cleaving enzyme phosphatase, producing ELF 97 alcohol (ELFA), a fluorescent-insoluble product. A high percentage of the cultured cells were labeled with ELFA (the ratio of ELFA spots to total DAPI counts in P-limited cultures ranged from 26 to 100%, depending on the strain). In contrast, this ratio never exceeded 0.01% in Mediterranean samples, even when P was demonstrated to be a significant limiting factor.
- The fluorogenic substrate Enzyme-Labeled Fluorescence 97 (ELF-P) is hydrolyzed by the P-cleaving enzyme phosphatase, producing ELF 97 alcohol (ELFA), a fluorescent-insoluble product. A high percentage of the cultured cells were labeled with ELFA (the ratio of ELFA spots to total DAPI counts in P-limited cultures ranged from 26 to 100%, depending on the strain). In contrast, this ratio never exceeded 0.01% in Mediterranean samples, even when P was demonstrated to be a significant limiting factor. (en)
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Title
| - Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea
- Aktivita alkalických fosfatáz mořských bakterií studovaná pomocí substrátu ELF97: úspěchy a meze použitelnosti metody ve fosforem limitovaném Středozemním moři (cs)
- Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea (en)
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skos:prefLabel
| - Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea
- Aktivita alkalických fosfatáz mořských bakterií studovaná pomocí substrátu ELF97: úspěchy a meze použitelnosti metody ve fosforem limitovaném Středozemním moři (cs)
- Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea (en)
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skos:notation
| - RIV/60077344:_____/08:00317620!RIV09-AV0-60077344
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/08:00317620
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - marine bacteria; alkaline phosphatase; ELF97 phosphatase substrate (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Aquatic Microbial Ecology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Nedoma, Jiří
- Van Wambeke, F.
- Duhamel, S.
- Lebaron, P.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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