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Description
| - Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake Čertovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphatase activity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer.
- Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake Čertovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphatase activity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer. (en)
- V acidifikovaném Čertově jezeře byla pomocí fluorogenního substrátu ELF97měřena za použití pomocí analýzy obrazu aktivita kyselých fosfatáz vázaných na povrch bakteriálních buněk. Najvyšší aktivita byla zjištěna u specifického morfotypu zahnutých buněk, jejichž podíl na biomase bakterioplanktonu činil v sezonním průměru 4%, zatímco jejich podíl na aktivitě enzymu byl 31%. Vláknité bakterie tvořily naopak 85% biomasy, ale jejich podíl na aktivitě enzymu byl jen 45%. Specifická aktivita vztažená na biomasu byla nejvyšší na jaře a snižovala se v průběhu sezony, byla nejvyšší u zahnutých buněk, střední u kokálních a tyčinkovitých buněk, a nejnižší u vláken. (cs)
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Title
| - Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake
- Specifická aktivita fosfatázy vázané na povrch buněk u různých morfotypů bakterioplanktonu v acidifikovaném horském jezeře (cs)
- Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake (en)
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skos:prefLabel
| - Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake
- Specifická aktivita fosfatázy vázané na povrch buněk u různých morfotypů bakterioplanktonu v acidifikovaném horském jezeře (cs)
- Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake (en)
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skos:notation
| - RIV/60077344:_____/06:00042946!RIV07-AV0-60077344
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(IAA6017202), V, Z(AV0Z60170517)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/06:00042946
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - alkaline phosphatase; bacterial morphorypes; acidified lake (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Environmental Microbiology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Nedoma, Jiří
- Vrba, Jaroslav
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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