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rdf:type
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Description
| - A relative simple method for the simultaneous determination of epinephrine, norepinephrine and dopamine in urine is presented. We have used a high-performance liquid chromatography (HPLC) with coulometric electrochemical detection. Urine samples pretreatment by a solid-phase extraction were injected directly into the HPLC system and were eluted isocratically on a Discovery HS C18, 250 x 4 mm i.d., 5 um, analytical column. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked urine samples were at intervals 78.2-88.0% for epinephrine, 80.0-93.0% for norepinephrine, and 96.4-107.2% for dopamine. The linear range is 2-200 ug l-1, with a detection limit of 0.6 ug l-1 (signal-to-noise ratio = 3) for epinephrine, 5-500 ug l-1, with a detection limit of 1.5 ug l-1 for norepinephrine, and 20-2000 ug l-1, with a detection limit of 6 ug l-1 for dopamine.
- A relative simple method for the simultaneous determination of epinephrine, norepinephrine and dopamine in urine is presented. We have used a high-performance liquid chromatography (HPLC) with coulometric electrochemical detection. Urine samples pretreatment by a solid-phase extraction were injected directly into the HPLC system and were eluted isocratically on a Discovery HS C18, 250 x 4 mm i.d., 5 um, analytical column. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked urine samples were at intervals 78.2-88.0% for epinephrine, 80.0-93.0% for norepinephrine, and 96.4-107.2% for dopamine. The linear range is 2-200 ug l-1, with a detection limit of 0.6 ug l-1 (signal-to-noise ratio = 3) for epinephrine, 5-500 ug l-1, with a detection limit of 1.5 ug l-1 for norepinephrine, and 20-2000 ug l-1, with a detection limit of 6 ug l-1 for dopamine. (en)
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Title
| - Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection
- Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection (en)
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skos:prefLabel
| - Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection
- Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection (en)
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skos:notation
| - RIV/00216275:25310/13:39896727!RIV14-MSM-25310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/13:39896727
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - solid-phase extraction; coulometric electrochemical detection; liquid chromatography; dopamine; norepinephrine; epinephrine (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kanďár, Roman
- Drábková, Petra
- Štramová, Xenie
- Žáková, Pavla
- Bencová, Soňa
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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