About: Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells     Goto   Sponge   Distinct   Permalink

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Description
  • An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes. 2D analysis of the distances between centre of nucleus-to-centre of chromosome 8 territory revealed that sodium butyrate did not affect nuclear arrangem.
  • An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes. 2D analysis of the distances between centre of nucleus-to-centre of chromosome 8 territory revealed that sodium butyrate did not affect nuclear arrangem. (en)
  • Rozdílná jaderná poloha aberantních a normálních homologů chromozomu 8 v kontrolních a NaBt diferencovaných HT-29 buňkách. (cs)
Title
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells (en)
  • Rozdílná jaderná poloha aberantních a normálních homologů chromozomu 8 v kontrolních a NaBt diferencovaných HT-29 buňkách (cs)
skos:prefLabel
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells
  • Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells (en)
  • Rozdílná jaderná poloha aberantních a normálních homologů chromozomu 8 v kontrolních a NaBt diferencovaných HT-29 buňkách (cs)
skos:notation
  • RIV/00216224:14330/04:00009711!RIV08-AV0-14330___
http://linked.open.../vavai/riv/strany
  • 24-27
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAA5004306), P(IBS5004010), P(KSK5052113), P(ME 565), P(NC6987), Z(AV0Z5004920)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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  • 560561
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14330/04:00009711
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http://linked.open.../riv/klicovaSlova
  • cytometry (en)
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http://linked.open...ontrolniKodProRIV
  • [02930EC93C6D]
http://linked.open...v/mistoKonaniAkce
  • Brno
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • Biophysics of the Genome
http://linked.open...in/vavai/riv/obor
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http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Bártová, Eva
  • Kozubek, Stanislav
  • Harničarová, Andrea
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Masaryk University
https://schema.org/isbn
  • 80-210-3560-9
http://localhost/t...ganizacniJednotka
  • 14330
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