About: TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes     Goto   Sponge   Distinct   Permalink

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Description
  • We have isolated and characterized a new repetitive sequence, TAS49, from terminal restriction fragments of Nicotiana tomentosiformis genomic DNA by means of a modified vectorette approach. The TAS49 was found directly attached to telomeres of N. tabacum and one of its ancestors, N. tomentosiformis, and also at inner chromosome locations. No association with telomeres was detected neither in N. otophora nor in the second tobacco ancestor, N. sylvestris. PCR and Southern hybridization reveal similarities in the arrangement of TAS49 on the chromosomes of 9 species of the genus Nicotiana, implying its occurrence as a subunit of a conserved complex DNA repeat. TAS49 belongs to the family of dispersed repetitive sequences without features of transposons. The copy number of TAS49 varies widely in the genomes of 8 species analyzed being lowest in N. sylvestris, with 3300 copies per diploid genome. In N. tomentosiformis, TAS49 forms about 0.56% of the diploid genome, corresponding to 17400 copies. TAS49 units
  • We have isolated and characterized a new repetitive sequence, TAS49, from terminal restriction fragments of Nicotiana tomentosiformis genomic DNA by means of a modified vectorette approach. The TAS49 was found directly attached to telomeres of N. tabacum and one of its ancestors, N. tomentosiformis, and also at inner chromosome locations. No association with telomeres was detected neither in N. otophora nor in the second tobacco ancestor, N. sylvestris. PCR and Southern hybridization reveal similarities in the arrangement of TAS49 on the chromosomes of 9 species of the genus Nicotiana, implying its occurrence as a subunit of a conserved complex DNA repeat. TAS49 belongs to the family of dispersed repetitive sequences without features of transposons. The copy number of TAS49 varies widely in the genomes of 8 species analyzed being lowest in N. sylvestris, with 3300 copies per diploid genome. In N. tomentosiformis, TAS49 forms about 0.56% of the diploid genome, corresponding to 17400 copies. TAS49 units (en)
  • We have isolated and characterized a new repetitive sequence, TAS49, from terminal restriction fragments of Nicotiana tomentosiformis genomic DNA by means of a modified vectorette approach. The TAS49 was found directly attached to telomeres of N. tabacum and one of its ancestors, N. tomentosiformis, and also at inner chromosome locations. No association with telomeres was detected neither in N. otophora nor in the second tobacco ancestor, N. sylvestris. PCR and Southern hybridization reveal similarities in the arrangement of TAS49 on the chromosomes of 9 species of the genus Nicotiana, implying its occurrence as a subunit of a conserved complex DNA repeat. TAS49 belongs to the family of dispersed repetitive sequences without features of transposons. The copy number of TAS49 varies widely in the genomes of 8 species analyzed being lowest in N. sylvestris, with 3300 copies per diploid genome. In N. tomentosiformis, TAS49 forms about 0.56% of the diploid genome, corresponding to 17400 copies. TAS49 units (cs)
Title
  • TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes
  • TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes (en)
  • TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes (cs)
skos:prefLabel
  • TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes
  • TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes (en)
  • TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes (cs)
skos:notation
  • RIV/00216224:14310/99:00002246!RIV09-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(VS97032), Z(AV0Z5004920), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 728861
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/99:00002246
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • repetitive DNA sequence; subtelomere; plant; Nicotiana (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CA - Kanada
http://linked.open...ontrolniKodProRIV
  • [C5430EEC5F9A]
http://linked.open...i/riv/nazevZdroje
  • Genome
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 43
http://linked.open...iv/tvurceVysledku
  • Fajkus, Jiří
  • Horáková, Mirka
http://linked.open...n/vavai/riv/zamer
issn
  • 0831-2796
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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