About: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering     Goto   Sponge   Distinct   Permalink

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  • Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.
  • Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters. (en)
Title
  • Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering
  • Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering (en)
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  • Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering
  • Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering (en)
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  • RIV/00216224:14310/10:00040598!RIV11-MSM-14310___
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  • P(IAA401630901), P(LC06010), Z(MSM0021622412)
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  • 35
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  • 257086
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  • RIV/00216224:14310/10:00040598
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  • Enantioselectivity; Haloalkane Dehalogenases; protein engineering (en)
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  • DE - Spolková republika Německo
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  • [2B640E4C6038]
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  • Angewandte Chemie International Edition
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  • 49
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  • Brezovský, Jan
  • Chaloupková, Radka
  • Damborský, Jiří
  • Jeřábek, Petr
  • Koudeláková, Táňa
  • Nagata, Yuji
  • Prokop, Zbyněk
  • Štěpánková, Veronika
  • Sato, Yukari
  • Mozga, Tomáš
  • Florian, J.
  • Janssen, D,B.
  • Leewen, J.G.E.
  • Natsume, Rio
  • Senda, T.
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  • 000281316000011
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  • 1433-7851
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  • 14310
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