About: Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans. Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans. (en)
Title	Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans (en)
skos:prefLabel	Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans (en)
skos:notation	RIV/00216224:14310/08:00026509!RIV10-MSM-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0528), Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Nováková, Dana Sedláček, Ivo Švec, Pavel Kukletová, Martina Žáčková, Lenka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	366800
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/08:00026509
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Dental caries; (GTG)5-PCR; Identification; Automated ribotyping; Streptococcus mutans (en)
http://linked.open.../riv/klicoveSlovo	(GTG)5-PCR Identification Dental caries Streptococcus mutans Automated ribotyping
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[FE1F139A60A6]
http://linked.open...i/riv/nazevZdroje	Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Dental research centre
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	94
http://linked.open...iv/tvurceVysledku	Sedláček, Ivo Švec, Pavel Kukletová, Martina Nováková, Dana Žáčková, Lenka
http://linked.open...ain/vavai/riv/wos	000259670400008
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
issn	0003-6072
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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