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rdf:type
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Description
| - Activity of FLC gene (Flowering Locus C) plays a central role in the control of flowering time in the autonomous and vernalization pathways. FLC activity is negatively regulated by other genes of both pathways. The main goal of this work was to assigne influence of the six late-flowering genes (dn, L4, L5, L6-1, L6 a Spi) of the new methylation pathway on the FLC expression. Also the effect of developmental stages, vernalization and 5-azacytidine treatment on the level of FLC mRNA in rosette leaves was analysed. Moreover the tissue distribution of FLC transcript was determined. For this purpose, reverse transcription and PCR amplification of FLC was used. All six late-flowering mutants of the methylation pathway increased level of FLC mRNA relative to control lines S96 and Di-G. For analysis of the effect of developmental stages, vernalization and 5-azacytidine treatment the level of FLC mRNA was examined in rosette leaves, which were collected from plants (L4 and Spi) before and after flowering. FLC
- Activity of FLC gene (Flowering Locus C) plays a central role in the control of flowering time in the autonomous and vernalization pathways. FLC activity is negatively regulated by other genes of both pathways. The main goal of this work was to assigne influence of the six late-flowering genes (dn, L4, L5, L6-1, L6 a Spi) of the new methylation pathway on the FLC expression. Also the effect of developmental stages, vernalization and 5-azacytidine treatment on the level of FLC mRNA in rosette leaves was analysed. Moreover the tissue distribution of FLC transcript was determined. For this purpose, reverse transcription and PCR amplification of FLC was used. All six late-flowering mutants of the methylation pathway increased level of FLC mRNA relative to control lines S96 and Di-G. For analysis of the effect of developmental stages, vernalization and 5-azacytidine treatment the level of FLC mRNA was examined in rosette leaves, which were collected from plants (L4 and Spi) before and after flowering. FLC (en)
- Gen FLC (Flowering Locus C) má centrální roli v kontrole kvetení, na níž se podílí geny autonomní a vernalizační dráhy. Aktivita genu FLC je negativně regulována geny obou těchto drah. Hlavním cílem této práce bylo stanovit vliv šesti genů pro pozdní kvetení (dn, L4, L5, L6-1, L6 a Spi) zařazených do nové metylační dráhy na expresi genu FLC. Dále byl analýzou růžicových listů zkoumán také vliv vývojových stadií, vernalizace a 5-azacytidinu na úroveň hladiny mRNA genu FLC. Vedle toho byla zkoumána též distribuce transkriptu genu FLC v jednotlivých rostlinných pletivech. Pro tento účel byla použita metoda zpětné transkripce a PCR amplifikace produktu genu FLC. U všech šesti pozdně kvetoucích mutantů metylační dráhy byla zjištěna zvýšená hladina mRNA genu FLC vzhledem ke kontrolním liniím S96 a Di-G. Pro analýzu vlivu vývojových stadií, vernalizace a 5-azacytidinu byla hladina mRNA zkoumána v růžicových listech, které byly získány z rostlin (L4 a Spi) před a po kvetení. Bylo zjištěno, že hladina mRNA gen (cs)
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Title
| - Influence of methylation pathway genes on FLC expression in Arabidopsis thaliana.
- Influence of methylation pathway genes on FLC expression in Arabidopsis thaliana. (en)
- Vliv genů metylační dráhy na expresi genu FLC u Arabidopsis thaliana (cs)
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skos:prefLabel
| - Influence of methylation pathway genes on FLC expression in Arabidopsis thaliana.
- Influence of methylation pathway genes on FLC expression in Arabidopsis thaliana. (en)
- Vliv genů metylační dráhy na expresi genu FLC u Arabidopsis thaliana (cs)
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skos:notation
| - RIV/00216224:14310/04:00011539!RIV08-GA0-14310___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/04:00011539
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Arabidopsis; flowering; methylation pathway; FLC gene (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - 15th International Conference on Arabidopsis Research
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Balková, Simona
- Lízal, Pavel
- Relichová, Jiřina
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
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http://localhost/t...ganizacniJednotka
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