About: Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)     Goto   Sponge   Distinct   Permalink

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Description
  • The second internal transcribed spacer (ITS2) of the ribosomal RNA genes of Diplozoon paradoxum and Paradiplozoon nagibinae were amplified and sequenced. The PCR product of D. paradoxum was bigger (840bp) than that of P. nagibinae (820bp). There was no intraspecific variability recorded in sequences from either species. Sequence comparisons and ITS2 RFLP pattern of eight European diplozoid species aimed to resolve their identification and amends the previous studies. Restriction fragment length polymorphism (RFLP) was used to distinguish both species from each other and from P. bliccae, P. homoion, P. megan, P. pavlovskii, P. sapae and Eudiplozoon nipponicum, using restriction enzymes AluI, HaeIII, HinfI, RsaI and SphI. The criteria for morphological identification of eight European diplozoids are also included with the main morphological characters of clamps, trapeze spur and anterior joining sclerites of eight diplozoid species being illustrated. Combination of the shape and comparison of length of
  • The second internal transcribed spacer (ITS2) of the ribosomal RNA genes of Diplozoon paradoxum and Paradiplozoon nagibinae were amplified and sequenced. The PCR product of D. paradoxum was bigger (840bp) than that of P. nagibinae (820bp). There was no intraspecific variability recorded in sequences from either species. Sequence comparisons and ITS2 RFLP pattern of eight European diplozoid species aimed to resolve their identification and amends the previous studies. Restriction fragment length polymorphism (RFLP) was used to distinguish both species from each other and from P. bliccae, P. homoion, P. megan, P. pavlovskii, P. sapae and Eudiplozoon nipponicum, using restriction enzymes AluI, HaeIII, HinfI, RsaI and SphI. The criteria for morphological identification of eight European diplozoids are also included with the main morphological characters of clamps, trapeze spur and anterior joining sclerites of eight diplozoid species being illustrated. Combination of the shape and comparison of length of (en)
  • ITS2 rDNA byla sekvenovana pro druhy Diplozoon paradoxum a Paradiplozoon nagibinae. PCR produkt D. paradoxum byl vetsi (840bp) nez pro P. nagibinae (820bp). Nebyla nalezena vnitrodruhova variabilita v sekvenci obou druhu. Porovnani sekvenci a vysledku RFLP ITS prokazalo existenci osmi druhu evropskych diplozoonu. (cs)
Title
  • Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)
  • Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2) (en)
  • Druhova identifikace evropskych diplozoonu (Diplozoidae, Monogenea) s pouzitim RFLP ITS2 rDNA (cs)
skos:prefLabel
  • Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)
  • Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2) (en)
  • Druhova identifikace evropskych diplozoonu (Diplozoidae, Monogenea) s pouzitim RFLP ITS2 rDNA (cs)
skos:notation
  • RIV/00216224:14310/04:00009908!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 817-822
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 143100010)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 567151
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/04:00009908
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • diplozoids; ITS2; genetic markers; maximum likelihood (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [6E3324239389]
http://linked.open...i/riv/nazevZdroje
  • Journal of Parasitology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 90
http://linked.open...iv/tvurceVysledku
  • Koubková, Božena
  • Matějusová, Iveta
  • Cunningham, Carey O.
http://linked.open...n/vavai/riv/zamer
issn
  • 0022-3395
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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