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  • Using a genomic approach, two Arabidopsis thaliana genes coding for candidate myb-like telomere bnding proteins were cloned and expressed in E. coli. Both proteins, termed as AtTBP2 and AtTBP3, contain a single Myb-like DNA-binding domain at the N-terminus, and a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. Gel-retardation assays showed that each of the two proteins is able to bind G-rich strand and double-strand DNA of plant telomeric sequence with the affinity proportional to a number of telomeric repeats.
  • Using a genomic approach, two Arabidopsis thaliana genes coding for candidate myb-like telomere bnding proteins were cloned and expressed in E. coli. Both proteins, termed as AtTBP2 and AtTBP3, contain a single Myb-like DNA-binding domain at the N-terminus, and a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. Gel-retardation assays showed that each of the two proteins is able to bind G-rich strand and double-strand DNA of plant telomeric sequence with the affinity proportional to a number of telomeric repeats. (en)
  • Using a genomic approach, two Arabidopsis thaliana genes coding for candidate myb-like telomere bnding proteins were cloned and expressed in E. coli. Both proteins, termed as AtTBP2 and AtTBP3, contain a single Myb-like DNA-binding domain at the N-terminus, and a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. Gel-retardation assays showed that each of the two proteins is able to bind G-rich strand and double-strand DNA of plant telomeric sequence with the affinity proportional to a number of telomeric repeats. (cs)
Title
  • Characterisation of two Arabidopsis thaliana myb-like proteins showing affinity to telomeric DNA sequence
  • Characterisation of two Arabidopsis thaliana myb-like proteins showing affinity to telomeric DNA sequence (en)
  • Charakterizace dvou myb-like proteinů z Arabidopsis thaliana, které vykazují afinitu k telomerickým sekvencím (cs)
skos:prefLabel
  • Characterisation of two Arabidopsis thaliana myb-like proteins showing affinity to telomeric DNA sequence
  • Characterisation of two Arabidopsis thaliana myb-like proteins showing affinity to telomeric DNA sequence (en)
  • Charakterizace dvou myb-like proteinů z Arabidopsis thaliana, které vykazují afinitu k telomerickým sekvencím (cs)
skos:notation
  • RIV/00216224:14310/03:00008351!RIV08-MSM-14310___
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  • 33
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  • P(GA204/02/0027), Z(MSM 143100008)
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  • 600898
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  • RIV/00216224:14310/03:00008351
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  • myb-like proteins; Arabidopsis thaliana (en)
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  • [FDBD802DDB28]
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  • Brno
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  • Brno
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  • VII. Pracovní setkání biochemiků a molekulárních biologů
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  • Rotková, Gabriela
  • Fajkus, Jiří
  • Krejčí, Lumír
  • Procházková Schrumpfová, Petra
  • Kuchař, Milan
  • Kunická, Zuzana
  • Skříšovská, Lenka
  • Vrtíková Kubičárová, Tatiana
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  • Masarykova univerzita v Brně
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  • 80-210-3053-4
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  • 14310
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