About: Spectrin-like protein in yeasts     Goto   Sponge   Distinct   Permalink

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Description
  • Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast speci
  • Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast speci (en)
  • Spectrin is the principal component of a system of structural proteins that are associated with the plasma membrane cytoplasmic surface. Its structure and function has been best characterized in mammalian erythrocytes (1). Proteins related to the a- and b-erythrocyte spectrin have been detected in almost all vertebrate tissues, and also in plants (2) and hyphal fungae (3). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies was used for spectrin immunodetection. Band at 220 kDa in cells and protoplasts extracts of both yeast strains was detected. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast speci (cs)
Title
  • Spectrin-like protein in yeasts
  • Spectrin-like protein in yeasts (en)
  • Spectrin-like protein in yeasts (cs)
skos:prefLabel
  • Spectrin-like protein in yeasts
  • Spectrin-like protein in yeasts (en)
  • Spectrin-like protein in yeasts (cs)
skos:notation
  • RIV/00216224:14110/02:00006734!RIV08-GA0-14110___
http://linked.open.../vavai/riv/strany
  • 36
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA204/00/0394), P(GA204/99/D025)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 664539
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/02:00006734
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • actin; spectrin; yeast; cytoskeleton (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [2FB95E9A60B7]
http://linked.open...v/mistoKonaniAkce
  • 29.-31.5.2002 Smolenice, Slovensko
http://linked.open...i/riv/mistoVydani
  • Bratislava
http://linked.open...i/riv/nazevZdroje
  • Sborník XXX th Annual Conference on yeasts
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Slaninová, Iva
  • Svoboda, Augustin
  • Holubářová, Alena
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Československá mikrobiologická společnost
http://localhost/t...ganizacniJednotka
  • 14110
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