About: Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA

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About: Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA Goto Sponge Distinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1002/cplu.201402194
Description	4-Hydroxy-3-methoxyphenyl (MOP) and 2,3-dihydrobenzofuran-5-yl (DHB) groups were tested as potential new oxidizable labels for electrochemical detection of DNA. The corresponding 5-aryl-cytosine and 7-aryl-7-deazaadenine 2-deoxyribonucleoside triphosphates (dNTPs) were prepared by the Suzuki coupling of halogenated dNTPs or by triphosphorylation of modified nucleosides, and were incorporated enzymatically into DNA by polymerase. In parallel, the electrochemical behavior of the labeled nucleosides, nucleotides, and DNA was studied to show that most of them give analytically useful signals of oxidation on the carbon electrode. Particularly, MOP appears to be a useful oxidizable DNA label since it offers independent detection of the modified DNA. 4-Hydroxy-3-methoxyphenyl (MOP) and 2,3-dihydrobenzofuran-5-yl (DHB) groups were tested as potential new oxidizable labels for electrochemical detection of DNA. The corresponding 5-aryl-cytosine and 7-aryl-7-deazaadenine 2-deoxyribonucleoside triphosphates (dNTPs) were prepared by the Suzuki coupling of halogenated dNTPs or by triphosphorylation of modified nucleosides, and were incorporated enzymatically into DNA by polymerase. In parallel, the electrochemical behavior of the labeled nucleosides, nucleotides, and DNA was studied to show that most of them give analytically useful signals of oxidation on the carbon electrode. Particularly, MOP appears to be a useful oxidizable DNA label since it offers independent detection of the modified DNA. (en)
Title	Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA (en)
skos:prefLabel	Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA (en)
skos:notation	RIV/00216208:11310/14:10286209!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GBP206/12/G151)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hocek, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	28695
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10286209
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	redox labeling; polymerase chain reactions; nucleotides; electrochemistry; DNA (en)
http://linked.open.../riv/klicoveSlovo	DNA electrochemistry nucleotides redox labeling polymerase chain reactions
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[0F255B6EB6AA]
http://linked.open...i/riv/nazevZdroje	ChemPlusChem
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of novel approaches to bioanalysis and molecular diagnostics
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Balintová, Jana Fojta, Miroslav Havran, Luděk Hocek, Michal Pohl, Radek Simonova, Anna
http://linked.open...ain/vavai/riv/wos	000345633900005
issn	2192-6506
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/cplu.201402194
http://localhost/t...ganizacniJednotka	11310

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