About: Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin

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About: Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin Goto Sponge Distinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1007/s12010-012-9772-y
Description	Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of alpha-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. alpha-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown. Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of alpha-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. alpha-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown. (en)
Title	Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin (en)
skos:prefLabel	Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin (en)
skos:notation	RIV/00216208:11310/12:10126758!RIV13-MSM-11310___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA203/09/0857), P(LC06044), Z(AV0Z40500505), Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Tichá, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	147748
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10126758
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	proteomics; microspheres; digestion; trypsin; porcine pepsin A; magnetic bead cellulose; immobilized alpha-chymotrypsin; human gastric juice; enzyme immobilization (en)
http://linked.open.../riv/klicoveSlovo	proteomics enzyme immobilization human gastric juice immobilized alpha-chymotrypsin magnetic bead cellulose porcine pepsin A digestion microspheres trypsin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A1B1748358E6]
http://linked.open...i/riv/nazevZdroje	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Experimental Hematology New analytical approaches for identification of proteins with significant attributes for virulence and pathogenity of bacteria
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	168
http://linked.open...iv/tvurceVysledku	Horák, Daniel Tichá, Marie Lenfeld, Jiří Kučerová, Zdenka Přikryl, Petr
http://linked.open...ain/vavai/riv/wos	000309224300007
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	0273-2289
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12010-012-9772-y
http://localhost/t...ganizacniJednotka	11310

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