About: NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.ejcb.2012.05.001
Description	The aim of this study was to analyze the potential role of NG2 chondroitin sulfate proteoglycan in amoeboid morphology and invasiveness of cancer cells. In the highly metastatic arnoeboid cell lines A3 and A375M2, siRNA-mediated down-regulation of NG2 induced an amoeboid-mesenchymal transition associated with decreased invasiveness in 3D collagen and inactivation of the GTPase Rho. Conversely, the expression of NG2 in mesenchymal sarcoma K2 cells as well as in A375M2 cells resulted in an enhanced amoeboid phenotype associated with increased invasiveness and elevated Rho-GTP levels. Remarkably, the amoeboid-mesenchymal transition in A375M2 cells triggered by NG2 dovin-regulation was associated with increased extracellular matrix-degrading ability, although this was not sufficient to compensate for the decreased invasive capability caused by down-regulated Rho/ROCK signaling. Conversely, in K2 cells with overexpression of NG2, the ability to degrade the extracellular matrix was greatly reduced. Taken together, we suggest that NG2-mediated activation of Rho leading to effective amoeboid invasiveness is a possible mechanism through which NG2 could contribute to tumor cell invasion and metastasis. (C) 2012 Elsevier GmbH. All rights reserved. The aim of this study was to analyze the potential role of NG2 chondroitin sulfate proteoglycan in amoeboid morphology and invasiveness of cancer cells. In the highly metastatic arnoeboid cell lines A3 and A375M2, siRNA-mediated down-regulation of NG2 induced an amoeboid-mesenchymal transition associated with decreased invasiveness in 3D collagen and inactivation of the GTPase Rho. Conversely, the expression of NG2 in mesenchymal sarcoma K2 cells as well as in A375M2 cells resulted in an enhanced amoeboid phenotype associated with increased invasiveness and elevated Rho-GTP levels. Remarkably, the amoeboid-mesenchymal transition in A375M2 cells triggered by NG2 dovin-regulation was associated with increased extracellular matrix-degrading ability, although this was not sufficient to compensate for the decreased invasive capability caused by down-regulated Rho/ROCK signaling. Conversely, in K2 cells with overexpression of NG2, the ability to degrade the extracellular matrix was greatly reduced. Taken together, we suggest that NG2-mediated activation of Rho leading to effective amoeboid invasiveness is a possible mechanism through which NG2 could contribute to tumor cell invasion and metastasis. (C) 2012 Elsevier GmbH. All rights reserved. (en)
Title	NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells (en)
skos:prefLabel	NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells (en)
skos:notation	RIV/00216208:11310/12:10126706!RIV13-MSM-11310___
http://linked.open...avai/riv/aktivita	I S Z
http://linked.open...avai/riv/aktivity	I, S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	11-12
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Brábek, Jan Rösel, Daniel Kratochvílová, Magdalena Paňková, Daniela Jobe, Njainday
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	154216
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10126706
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Invadopodia; Rho; Mesenchymal; Amoeboid; Invasiveness; NG2 (en)
http://linked.open.../riv/klicoveSlovo	Invasiveness Amoeboid Invadopodia Mesenchymal NG2 Rho
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[7B290B0F15CC]
http://linked.open...i/riv/nazevZdroje	European Journal of Cell Biology
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	91
http://linked.open...iv/tvurceVysledku	Brábek, Jan Rösel, Daniel Buccione, Roberto Jobe, Njainday Kratochvílová, Magdalena Paňková, Daniela
http://linked.open...ain/vavai/riv/wos	000311775000018
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0171-9335
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ejcb.2012.05.001
http://localhost/t...ganizacniJednotka	11310

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