About: Fluorescence Spectroscopy as a Tool for Investigating the Self-Organized Polyelectrolyte Systems

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1007/12_2010_56
Description	We have employed laser-induced liquid bead ion desorption mass spectroscopy (LILBID MS) to study the solution behavior of Pseudomonas aeruginosa azurin as well as two mutants and corresponding Re-labeled derivatives containing a Re(CO)3(4,7-dimethyl-1,10-phenanthroline)+ chromophore appended to a surface histidine. LILBID spectra show broad oligomer distributions whose particular patterns depend on the solution composition (pure H2O, 20MINUS SIGN 30 mM NaCl, 20 and 50 mM NaPi or NH4Pi at pH = 7). The distribution maximum shifts to smaller oligomers upon decreasing the azurin concentration and increasing the buffer concentration. Oligomerization is less extensive for native azurin than its mutants. The oligomerization propensities of unlabeled and Re-labeled proteins are generally comparable, and only Re126 shows some preference for the dimer that persists even in highly diluted solutions. We have employed laser-induced liquid bead ion desorption mass spectroscopy (LILBID MS) to study the solution behavior of Pseudomonas aeruginosa azurin as well as two mutants and corresponding Re-labeled derivatives containing a Re(CO)3(4,7-dimethyl-1,10-phenanthroline)+ chromophore appended to a surface histidine. LILBID spectra show broad oligomer distributions whose particular patterns depend on the solution composition (pure H2O, 20MINUS SIGN 30 mM NaCl, 20 and 50 mM NaPi or NH4Pi at pH = 7). The distribution maximum shifts to smaller oligomers upon decreasing the azurin concentration and increasing the buffer concentration. Oligomerization is less extensive for native azurin than its mutants. The oligomerization propensities of unlabeled and Re-labeled proteins are generally comparable, and only Re126 shows some preference for the dimer that persists even in highly diluted solutions. (en)
Title	Fluorescence Spectroscopy as a Tool for Investigating the Self-Organized Polyelectrolyte Systems Fluorescence Spectroscopy as a Tool for Investigating the Self-Organized Polyelectrolyte Systems (en)
skos:prefLabel	Fluorescence Spectroscopy as a Tool for Investigating the Self-Organized Polyelectrolyte Systems Fluorescence Spectroscopy as a Tool for Investigating the Self-Organized Polyelectrolyte Systems (en)
skos:notation	RIV/00216208:11310/11:10099034!RIV13-GA0-11310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA203/07/0659), P(IAA400400621), P(IAA401110702), P(KJB401110701), S, Z(MSM0021620857)
http://linked.open...iv/cisloPeriodika	241
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Limpouchová, Zuzana Procházka, Karel Kuldová, Jitka Košovan, Peter Uhlík, Filip Uchman, Mariusz Marcin Štěpánek, Miroslav Matějíček, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	200026
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/11:10099034
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	pseudomonas aeruginosa azurin solutions; oligomers; mass spectrometry (en)
http://linked.open.../riv/klicoveSlovo	oligomers pseudomonas aeruginosa azurin solutions mass spectrometry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[F4482511D4C8]
http://linked.open...i/riv/nazevZdroje	Advances in Polymer Science
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Multidisciplinary study of aqueous solutions of branched polyelectrolytes Molecular Dynamics Simulations of Annealed Polyelectrolytes DNA Condensation: Monte-Carlo Simulations, Light Scattering, Fluorescence Correlation Spectroscopy in vitro and in vivo. Associating Amphiphilic Miktoarm and Heteroarm Polyelectrolytes
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	2011
http://linked.open...iv/tvurceVysledku	Hof, Martin Matějíček, Pavel Uhlík, Filip Košovan, Peter Kuldová, Jitka Limpouchová, Zuzana Procházka, Karel Šachl, Radek Humpolíčková, Jana Uchman, Mariusz Marcin Štěpánek, Miroslav
http://linked.open...ain/vavai/riv/wos	000298919000006
http://linked.open...n/vavai/riv/zamer	Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí
issn	0065-3195
number of pages	63 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/12_2010_56
http://localhost/t...ganizacniJednotka	11310

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