About: Modern bioanalysis of proteins by electrophoretic techniques

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1007/978-1-62703-977-2_28
Description	In 1957, protein rich in cysteine able to bind cadmium was isolated from horse kidney and named as metallothionein according to its structural properties. Further, this protein and metallothionein-like proteins have been found in tissues of other animal species, yeasts, fungi and plants. MT is as a potential cancer marker in the focus of interest, and its properties, functions, and behavior under various conditions are intensively studied. Our protocol describes separation of two major mammalian isoforms of MT (MT-1 and MT-2) using capillary electrophoresis (CE) coupled with UV detector. This protocol enables separation of MT isoforms and studying of their basic behavior as well as their quantification with detection limit in units of ng per μL. Sodium borate buffer (20 mM, pH 9.5) was optimized as a background electrolyte, and the separation was carried out in fused silica capillary with internal diameter of 75 μm and electric field intensity of 350 V/cm. Optimal detection wavelength was 254 nm. In 1957, protein rich in cysteine able to bind cadmium was isolated from horse kidney and named as metallothionein according to its structural properties. Further, this protein and metallothionein-like proteins have been found in tissues of other animal species, yeasts, fungi and plants. MT is as a potential cancer marker in the focus of interest, and its properties, functions, and behavior under various conditions are intensively studied. Our protocol describes separation of two major mammalian isoforms of MT (MT-1 and MT-2) using capillary electrophoresis (CE) coupled with UV detector. This protocol enables separation of MT isoforms and studying of their basic behavior as well as their quantification with detection limit in units of ng per μL. Sodium borate buffer (20 mM, pH 9.5) was optimized as a background electrolyte, and the separation was carried out in fused silica capillary with internal diameter of 75 μm and electric field intensity of 350 V/cm. Optimal detection wavelength was 254 nm. (en)
Title	Modern bioanalysis of proteins by electrophoretic techniques Modern bioanalysis of proteins by electrophoretic techniques (en)
skos:prefLabel	Modern bioanalysis of proteins by electrophoretic techniques Modern bioanalysis of proteins by electrophoretic techniques (en)
skos:notation	RIV/00216208:11130/14:10294011!RIV15-MSM-11130___
http://linked.open...avai/riv/aktivita	V
http://linked.open...avai/riv/aktivity	V
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Eckschlager, Tomáš
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 2. lékařská fakulta
http://linked.open...dnocenehoVysledku	30027
http://linked.open...ai/riv/idVysledku	RIV/00216208:11130/14:10294011
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Electrochemistry; Chip electrophoresis; Capillary electrophoresis; Gel electrophoresis; Metallomics (en)
http://linked.open.../riv/klicoveSlovo	Metallomics Capillary electrophoresis Electrochemistry Gel electrophoresis Chip electrophoresis
http://linked.open...ontrolniKodProRIV	[AA22DC2AE7A9]
http://linked.open...i/riv/mistoVydani	New York City
http://linked.open...vEdiceCisloSvazku	Methods in Molecular Biology, Vol. 1129
http://linked.open...i/riv/nazevZdroje	Protein Downstream Processing
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...v/pocetStranKnihy	555 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Eckschlager, Tomáš Hubálek, Jaromír Křížková, Soňa Masařík, Michal Ryvolová, Markéta Zítka, Ondřej Kízek, René
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-977-2_28
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-62703-976-5
http://localhost/t...ganizacniJednotka	11130

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