About: Minimizing detection errors in single molecule localization microscopy

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-19-4-3226}
Description	Fluorescence microscopy using single molecule imaging and localization (PALM,STORM, and similar approaches) has quickly been adopted as a convenient method for obtaining multicolor, 3D superresolution images of biological samples. Using an approach based on extensive Monte Carlo simulations, we examined the performance of various noise reducing filters required for the detection of candidate molecules. We determined a suitable noise reduction method and derived an optimal, nonlinear threshold which minimizes detection errors introduced by conventional algorithms. We also present a new technique for visualization of single molecule localization microscopy data based on adaptively jittered 2D histograms. We have used our new methods to image both Atto565-phalloidin labeled actin in fibroblast cells, and mCitrine-erbB3 expressed in A431 cells. The enhanced methods developed here were crucial in processing the data we obtained from these samples,as the overall signal to noise ratio was quite low. Fluorescence microscopy using single molecule imaging and localization (PALM,STORM, and similar approaches) has quickly been adopted as a convenient method for obtaining multicolor, 3D superresolution images of biological samples. Using an approach based on extensive Monte Carlo simulations, we examined the performance of various noise reducing filters required for the detection of candidate molecules. We determined a suitable noise reduction method and derived an optimal, nonlinear threshold which minimizes detection errors introduced by conventional algorithms. We also present a new technique for visualization of single molecule localization microscopy data based on adaptively jittered 2D histograms. We have used our new methods to image both Atto565-phalloidin labeled actin in fibroblast cells, and mCitrine-erbB3 expressed in A431 cells. The enhanced methods developed here were crucial in processing the data we obtained from these samples,as the overall signal to noise ratio was quite low. (en)
Title	Minimizing detection errors in single molecule localization microscopy Minimizing detection errors in single molecule localization microscopy (en)
skos:prefLabel	Minimizing detection errors in single molecule localization microscopy Minimizing detection errors in single molecule localization microscopy (en)
skos:notation	RIV/00216208:11110/11:10550!RIV12-GA0-11110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA304/09/1047), P(LC535), Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Raška, Ivan Křížek, Pavel Hagen, Guy Michael
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	212623
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/11:10550
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence microscopy; image processing; fluorescent-probes; superresolution; nanoscale; cells (en)
http://linked.open.../riv/klicoveSlovo	image processing superresolution cells nanoscale fluorescent-probes fluorescence microscopy
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[02AD79BF644C]
http://linked.open...i/riv/nazevZdroje	Optics Express
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions Development of the programmable array microscope for study 4D cellular dynamics
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Raška, Ivan Hagen, Guy Michael Křížek, Pavel
http://linked.open...ain/vavai/riv/wos	000288860000041
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	1094-4087
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	11110

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