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Description
| - S cílem posoudit riziko průniku virulentních kmenů Staphylococcus epidermidis do potravního řetězce byly genotypově (icaAB geny) a fenotypově (in vitro tvorba biofilmu, růst na Congo red agaru; CRA) vyšetřovány izoláty ze vzorků „farm to fork“ (182 izolátů ze 2779 vzorků). Kontaminace masa a mléka byla významně vyšší (P<0.01) než kontaminace surovyn. Kontaminace kontaktních površích byla významně nižší v masných provozech než v mlékárnách: 5.8% oproti 15.5% kontaminovaných vzorků (P<0.01). ica geny, které podmiňují tvorbu biofilmu, byly prokázány u 20 izolátů, které současně vykazovaly typický růst na CRA. 2 ica operon-negativní izoláty tvořily in vitro biofilm, avšak pravděpodobné na základě jiného mechanismu než na ica závislém. Povrchy v mlékárnách a mléčné výrobky byly četněji kontaminovány ica operon-pozitivními kmeny (5.8% a 1.2% vzorků) než ostatní typy vzorků (0-0.6% vzorků). Z poznatku plyne především požadavek větších nároků na hygienu specifického prostředí mlékárenských závodů. (cs)
- Isolates from %22farm to fork%22 samples (182 isolates from 2779 samples) were examined genotypically (icaAB geny) and phenotypically (in vitro biofilm formation, typical growth on Congo red agar; CRA) with the aim to assess the risk of penetration of virulent strains of Staphylococcus epidermidis into the food chain. The contamination of meat and milk products with S. epidermidis was significantly higher in comparison with raw materials. Contamination of contact surfaces in the meat processing plants was significantly lower than of contact surfaces in the dairy plants. The ica genes, which precondition the biofilm formation, were concurrently detected in 20 isolates that also showed a typical growth on CRA. Two ica operon-negative isolates produced biofilm in vitro, however, perhaps by an ica-independent mechanism. The surfaces in the dairy plants and the milk products were more frequently contaminated with ica operon-positive strains (2.3 and 1.2 % samples) than the other sample types (0-0.6 % samples).
- Isolates from %22farm to fork%22 samples (182 isolates from 2779 samples) were examined genotypically (icaAB geny) and phenotypically (in vitro biofilm formation, typical growth on Congo red agar; CRA) with the aim to assess the risk of penetration of virulent strains of Staphylococcus epidermidis into the food chain. The contamination of meat and milk products with S. epidermidis was significantly higher in comparison with raw materials. Contamination of contact surfaces in the meat processing plants was significantly lower than of contact surfaces in the dairy plants. The ica genes, which precondition the biofilm formation, were concurrently detected in 20 isolates that also showed a typical growth on CRA. Two ica operon-negative isolates produced biofilm in vitro, however, perhaps by an ica-independent mechanism. The surfaces in the dairy plants and the milk products were more frequently contaminated with ica operon-positive strains (2.3 and 1.2 % samples) than the other sample types (0-0.6 % samples). (en)
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Title
| - The biofilm-positive Staphylococcus epidermidis isolates in raw materials, foodstuffs and on contact surfaces in processing plants
- Biofilm-pozitivní izoláty Staphylococcus epidermidis isolates v surovinách, potravinách a na kontaktních površích výrobních závodů (cs)
- The biofilm-positive Staphylococcus epidermidis isolates in raw materials, foodstuffs and on contact surfaces in processing plants (en)
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skos:prefLabel
| - The biofilm-positive Staphylococcus epidermidis isolates in raw materials, foodstuffs and on contact surfaces in processing plants
- Biofilm-pozitivní izoláty Staphylococcus epidermidis isolates v surovinách, potravinách a na kontaktních površích výrobních závodů (cs)
- The biofilm-positive Staphylococcus epidermidis isolates in raw materials, foodstuffs and on contact surfaces in processing plants (en)
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skos:notation
| - RIV/00027162:_____/08:#0000372!RIV09-MZE-00027162
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(QF4048), Z(MZE0002716201)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000372
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Staphylococcus epidermidis; food; biofilm (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Folia Microbiologica Prague
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Babák, Vladimír
- Holasová, Martina
- Schlegelová, Jarmila
- Dendis, M.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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