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rdf:type
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Description
| - V této práce jsme se zabývali tvorbou biofilmu u 94 terénních kmenů S. Typhimurium. Kmeny jsem testovali na produkci komponent, které jsou pro tvorbu biofilmu nezbytné, tedy fimbrií, celulózy a kapsulárního polysacharidu. Většina kmenů byla schopna tvořit biofilm. Výjimkou byly kmeny fágového typu DT2 pocházející z holubů. Kmeny nejvíce tvořící biofilm náležely k fágovému typu DT104 se Salmonella Genomic Island 1 (SGI1) ve svém genomu. Porovnání SGI1 pozitivních a negativních kmenů potvrdilo kladný vliv SGI1 na tvorbu biofilmu. Mimo to jsme u 5 kmenů prokázali alternativní strategii tvorby biofilmu založenou na nadprodukci kapsulárního polysacharidu. Pro mukoidní vzhled a tvorbu hnědých kolonií na Congo Red agaru jsem tyto kmeny označili jako SBAM morfotyp (Smooth Brown and Muciod). (cs)
- In this study we examined the extent of biofilm formation in field strains of Salmonella enterica serovar Typhimurium. Ninety-four field strains of S. Typhimurium were tested for their ability to form biofilm and components contributing to its formation. Most S. Typhimurium strains were highly capable of biofilm formation except for strains of phage type DT2 originating from pigeons. The most efficient biofilm forming strains were those of phage type DT104 positive for Salmonella genomic island 1 (SGI1). A comparison of SGI1 positive and negative strains indicated that biofilm formation of SGI1 positive strains was associated with the presence of this genomic island. Finally, in 5 strains we found an alternative strategy of biofilm formation independent of curli fimbriae and cellulose production but solely dependent on an overproduction of capsular polysaccharide. Due to a mucoid and brown appearance on Congo Red agar we designated these strains as SBAM (Smooth Brown and Mucoid) morphotype.
- In this study we examined the extent of biofilm formation in field strains of Salmonella enterica serovar Typhimurium. Ninety-four field strains of S. Typhimurium were tested for their ability to form biofilm and components contributing to its formation. Most S. Typhimurium strains were highly capable of biofilm formation except for strains of phage type DT2 originating from pigeons. The most efficient biofilm forming strains were those of phage type DT104 positive for Salmonella genomic island 1 (SGI1). A comparison of SGI1 positive and negative strains indicated that biofilm formation of SGI1 positive strains was associated with the presence of this genomic island. Finally, in 5 strains we found an alternative strategy of biofilm formation independent of curli fimbriae and cellulose production but solely dependent on an overproduction of capsular polysaccharide. Due to a mucoid and brown appearance on Congo Red agar we designated these strains as SBAM (Smooth Brown and Mucoid) morphotype. (en)
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Title
| - Biofilm formation in field strains of Salmonella enterica serovar Typhimurium: identification of a new colony morphology type and the role of SGI1 in biofilm formation
- Tvorba biofilmu terénními kmeny Salmonella enterica serovar Typhimurium: idnetifikace nového morfotypu a úloha SGI1 při tvorbě biofilmu (cs)
- Biofilm formation in field strains of Salmonella enterica serovar Typhimurium: identification of a new colony morphology type and the role of SGI1 in biofilm formation (en)
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skos:prefLabel
| - Biofilm formation in field strains of Salmonella enterica serovar Typhimurium: identification of a new colony morphology type and the role of SGI1 in biofilm formation
- Tvorba biofilmu terénními kmeny Salmonella enterica serovar Typhimurium: idnetifikace nového morfotypu a úloha SGI1 při tvorbě biofilmu (cs)
- Biofilm formation in field strains of Salmonella enterica serovar Typhimurium: identification of a new colony morphology type and the role of SGI1 in biofilm formation (en)
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skos:notation
| - RIV/00027162:_____/08:#0000349!RIV09-MZE-00027162
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1B44020), P(LC06030), Z(MZE0002716201)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000349
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Biofilm; Salmonella Typhimurium; curli fimbrie; DT104; SGI1 (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hradecká, Helena
- Malcová, Marcela
- Rychlík, Ivan
- Karpíšková, R.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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