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Description
| - The results of the study was the characterization of the course of antibody response after infection with App serotype 9 in piglets with different types and levels of antigen-specific immunity and the distinguishing of colostrum-derived antibodies from post-infection antibodies on the basis of their isotype characterization. The titres of serum and bronchoalveolar lavages (BALF) immunoglobulin IgG, IgM and IgA antibodies specific for LPS App9 were determined by isotype-specific indirect enzyme linked immunosorbent assays.In animals, that did not encounter App infection and did not passively ingest antigen-specific antibodies, antibody response was not recorded either in serum or in BALF. Piglets exposed to pathogen produced serum IgM and IgA in BALF, followed by IgG. Based on the assessment of specific IgM antibodies in sera and IgA antibodies in BALF, it is possible to detect subclinically infected animals, even in the presence of colostrum-derived antibodies (IgG).
- The results of the study was the characterization of the course of antibody response after infection with App serotype 9 in piglets with different types and levels of antigen-specific immunity and the distinguishing of colostrum-derived antibodies from post-infection antibodies on the basis of their isotype characterization. The titres of serum and bronchoalveolar lavages (BALF) immunoglobulin IgG, IgM and IgA antibodies specific for LPS App9 were determined by isotype-specific indirect enzyme linked immunosorbent assays.In animals, that did not encounter App infection and did not passively ingest antigen-specific antibodies, antibody response was not recorded either in serum or in BALF. Piglets exposed to pathogen produced serum IgM and IgA in BALF, followed by IgG. Based on the assessment of specific IgM antibodies in sera and IgA antibodies in BALF, it is possible to detect subclinically infected animals, even in the presence of colostrum-derived antibodies (IgG). (en)
- Výsledkem této studie byl popis protilátkové odpovědi na infekci App sérotyp 9, probíhající u selat s různým typem a úrovní antigen specifické imunity a dále rozlišení antigen-specifických kolostrálních protilátek od protilátek postinfekčních na základějejich izotypové charakteristiky. Nepřímou enzymatickou imunoanalýzou byly ve vzorcích séra a bronchoalveolárních laváží (BALF) měřeny hladiny IgG, IgM a IgA protilátek, specifických proti LPS App 9.U zvířat, která se nesetkala s infekcí App a pasivně nepřijala protilátky proti App, nebyla zaznamenána protilátková odpověď ani ve vzorcích séra ani v BALF. Na kontakt s patogenem selata zareagovala tvorbou IgM v séru a IgA v BALF, následovanou tvorbou IgG. Na základě sledování hladin IgM v séru a IgA v BALF je možné odlišit zvířata subklinicky infikovaná, a to i v době přítomnosti kolostrálních protilátek (IgG). (cs)
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Title
| - Diagnostic relevance of detection of antibody response to Actinobacillus pleuropneumoniae (App) infection in piglets
- Diagnostic relevance of detection of antibody response to Actinobacillus pleuropneumoniae (App) infection in piglets (en)
- Diagnostický význam sledování protilátkové odpovědi u aktinobacilové pleuropneumonie (App) selat (cs)
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skos:prefLabel
| - Diagnostic relevance of detection of antibody response to Actinobacillus pleuropneumoniae (App) infection in piglets
- Diagnostic relevance of detection of antibody response to Actinobacillus pleuropneumoniae (App) infection in piglets (en)
- Diagnostický význam sledování protilátkové odpovědi u aktinobacilové pleuropneumonie (App) selat (cs)
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skos:notation
| - RIV/00027162:_____/04:00011005!RIV/2005/MZE/M03005/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/04:00011005
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - bacterial infection;BALF;colostrum-derived antibodies;ELISA;mucosal immunity;post-infection antibodies;respiratory tract (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - 18th International Pig Veterinary Society Congress, June 27 - July 1, 2004, Hamburg
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
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