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Description
  • Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, ß- CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in a complex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS–PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, ß-, and ?-CDs were detected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme.
  • Cyclodextrins (CDs) are synthesized by the bacterial extracellular enzym cyclodextrin glycosyltransferase (CGTase, E.C. 2.4.1.19) from starch or starch derivatives via an inter- and intramolecural transglycosylation reaction. The production of ?- CD, ß- CD and ?- CD by CGTase from Paenibacillus macerans was studied, in regard to the effect of the source starch (amaranth, corn) on the yield of CDs. The CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB in a complex medium containing starch and partially purified by ammonium sulphate precipitation at 60% saturation. Apparent molecular weight as determined by SDS–PAGE was in the range from 105 to 114 kDa. All the commercially important ?-, ß-, and ?-CDs were detected chromatographically after hydrolysis of the corn and amaranth (Amaranthus cruentus) starch with the isolated enzyme. (en)
Title
  • Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)
  • Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea) (en)
skos:prefLabel
  • Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea)
  • Optimalization of production of cyclodextrin glycosyl transferase by Paenibacillus macerans strain and cyclodextrin formation (Abstract: Proceedings of The 5th Asian Cyclodextrin Conference (ACC2009). May 13-16, 2009. Busan, Korea) (en)
skos:notation
  • RIV/00027022:_____/09:00000129!RIV10-MSM-00027022
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B06173)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 331971
http://linked.open...ai/riv/idVysledku
  • RIV/00027022:_____/09:00000129
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cyclodextrin; fermentation; starch (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [6F0BFD26BCA4]
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Beran, Miloš
  • Urban, Marian
  • Adámek, Lubomír
  • Drahorád, Josef
  • Molík, Petr
  • Matušová, Krystina
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