About: Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler

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About: Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler Goto Sponge Distinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.vuzv.cz/sites/File/_privat/11128.pdf
Description	The goal of this work was to develop a protocol for rapid genotyping of A and G variants at the CSN2 locus and genotyping of T and C variants at the CSN3 locus in sheep breeds (Sumava and Valachian) by means of PCR and LightCycler analysis. The LightCycler technique combines rapid and efficient in vitro amplification of DNA in glass capillaries with melting curve analysis based on fluorescence resonance energy transfer for the sensitive detection of point mutation. The A variant had a greater frequency (Sumava, 0.778; Valachian, 0.835) than did variant G (Sumava, 0.222; Valachian, 0.165) in both sheep breeds. The CSN3 locus was found to be monomorphic, with no polymorphism identified in either population. The goal of this work was to develop a protocol for rapid genotyping of A and G variants at the CSN2 locus and genotyping of T and C variants at the CSN3 locus in sheep breeds (Sumava and Valachian) by means of PCR and LightCycler analysis. The LightCycler technique combines rapid and efficient in vitro amplification of DNA in glass capillaries with melting curve analysis based on fluorescence resonance energy transfer for the sensitive detection of point mutation. The A variant had a greater frequency (Sumava, 0.778; Valachian, 0.835) than did variant G (Sumava, 0.222; Valachian, 0.165) in both sheep breeds. The CSN3 locus was found to be monomorphic, with no polymorphism identified in either population. (en)
Title	Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler (en)
skos:prefLabel	Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler Technical note: A novel method for routine genotyping of the G allele of β-casein (CSN2) and T allele of κ-casein (CSN3) in a sheep population using LightCycler (en)
skos:notation	RIV/00027014:_____/11:#0001491!RIV12-MZE-00027014
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002701404)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Czerneková, Vladimíra Sztankóová, Zuzana Rychtářová, Jana Kyselová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	234309
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/11:#0001491
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	beta-casein, κ-casein, genetic polymorphism, LightCycler (en)
http://linked.open.../riv/klicoveSlovo	κ-casein LightCycler beta-casein genetic polymorphism
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[70CEECAAA65E]
http://linked.open...i/riv/nazevZdroje	Journal of Animal Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	89
http://linked.open...iv/tvurceVysledku	Czerneková, Vladimíra Sztankóová, Zuzana Kyselová, Jitka Rychtářová, Jana
http://linked.open...ain/vavai/riv/wos	000297368200001
http://linked.open...n/vavai/riv/zamer	Udržitelný rozvoj chovu hospodářských zvířat v evropském modelu multifunkčního zemědělství
issn	0021-8812
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.2527/jas.2011-4054

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