About: Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Microplate assays with 96 wells were optimized to screen proteolytic activities in mite homogenates. Whole-mite extracts of seven species of mites exhibited non-specific proteolytic activity in buffers from pH 2 to 12, and three peaks of highest activity at pH 3, 5-6, and 10 were distinguished. The reducing agent Tris(2-carboxyethyl) phosphine hydrochloride decreased general proteolytic activity on azocasein at pH 5 and 6. The results obtained on two non-specific substrates, azocasein and azoalbumin, showed highly different ranks of the species at pH 5 and 6. Evidence of the presence of proteolytic activities on all tested substrates and in all the tested mite homogenates suggests that the proteolytic activities may contribute to allergenicity. Poor or undetected hydrolytic activities of mite extracts toward substrates for keratin and collagen at digestive pH underline the importance of ecological interactions between mites and microorganisms in the utilization of such substrates. Microplate assays with 96 wells were optimized to screen proteolytic activities in mite homogenates. Whole-mite extracts of seven species of mites exhibited non-specific proteolytic activity in buffers from pH 2 to 12, and three peaks of highest activity at pH 3, 5-6, and 10 were distinguished. The reducing agent Tris(2-carboxyethyl) phosphine hydrochloride decreased general proteolytic activity on azocasein at pH 5 and 6. The results obtained on two non-specific substrates, azocasein and azoalbumin, showed highly different ranks of the species at pH 5 and 6. Evidence of the presence of proteolytic activities on all tested substrates and in all the tested mite homogenates suggests that the proteolytic activities may contribute to allergenicity. Poor or undetected hydrolytic activities of mite extracts toward substrates for keratin and collagen at digestive pH underline the importance of ecological interactions between mites and microorganisms in the utilization of such substrates. (en)
Title	Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites (en)
skos:prefLabel	Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites (en)
skos:notation	RIV/00027006:_____/10:00001292!RIV11-GA0-00027006
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP525/07/P253), P(OC09034), Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hubert, Jan Erban, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	251312
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/10:00001292
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protease; digestion; enzyme; allergen; synanthropic acaridid mite (en)
http://linked.open.../riv/klicoveSlovo	allergen enzyme protease digestion synanthropic acaridid mite
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E99DB710EF00]
http://linked.open...i/riv/nazevZdroje	Archives of Insect Biochemistry and Physiology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Proteins of bacterial origin occuring in the gut of synanthropic mites Acarcidial compounds targeted against midgut of synantropic mites
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	75
http://linked.open...iv/tvurceVysledku	Erban, Tomáš Hubert, Jan
http://linked.open...ain/vavai/riv/wos	000283778200004
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0739-4462
number of pages	20 (xsd:int)

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