About: Zpráva o řešení projektu QC 1336 za r. 2003

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Molecular markers have been succesfully to used to characterise genetic resources of many plant species and they were employed in marker assisted selection. New high throughput technologies have been introduced recently to speed up the analysis. Potential of cDNA arrays is evaluated using two different species: barley and hop. In RICP barley is evaluated using DNA and expression cDNA arrays. Several protocols for RNA/mRNA isolation were tested. Plasmid carrying inserts corresponding to cold indiced barley cDNA were isolated and insetrs were amplified. Protocol for amplification of insters from bacteria directly was introduced and adopted. The number of 96 amplification products were spotted on the glass slides and hybridized with Cy3, Cy5 labelled cDNA prepared from cold stressed barley seedling to optimise array technology. RNA quality was verified by RT qPCR. Ubiquitin was used as a control. It is apparent, that more comparators will be required. A set of 96 DNAs was prepared to test DNA/DNA b (en) češ češ (cs)
Title	Zpráva o řešení projektu QC 1336 za r. 2003 Report of the projet: QC 1336 2003 (en) Zpráva o řešení projektu QC 1336 za r. 2003 (cs)
skos:prefLabel	Zpráva o řešení projektu QC 1336 za r. 2003 Report of the projet: QC 1336 2003 (en) Zpráva o řešení projektu QC 1336 za r. 2003 (cs)
skos:notation	RIV/00027006:_____/04:####1146!RIV06-MZE-00027006
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QC1336)
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Ovesná, Jaroslava
http://linked.open.../riv/druhVysledku	V - Výzkumná zpráva
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	596258
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/04:####1146
http://linked.open...riv/jazykVysledku	cze - čeština
http://linked.open.../riv/klicovaSlova	DNA čipy, ječmen, diverzita, AFLP, SSR (en)
http://linked.open.../riv/klicoveSlovo	ječmen SSR AFLP diverzita DNA čipy
http://linked.open...ontrolniKodProRIV	[CBB1C5E31D8A]
http://linked.open...i/riv/mistoVydani	VURV Praha
http://linked.open...telVyzkumneZpravy	VURV Praha
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	Development and employment of new approaches for monitoring of genetic diversity and large-scale screening of genetic resources
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Ovesná, Jaroslava
http://linked.open...rzeVyzkumneZpravy	zprava
number of pages	78 (xsd:int)
https://schema.org/isbn	N

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