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  • Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution.
  • Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution. (en)
  • Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution. (cs)
Title
  • Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia
  • Molekulární podstata rezistence českých biotypů bytele metlatého vůči inhibitorům acetolaktát syntázy (cs)
  • Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia (en)
skos:prefLabel
  • Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia
  • Molekulární podstata rezistence českých biotypů bytele metlatého vůči inhibitorům acetolaktát syntázy (cs)
  • Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia (en)
skos:notation
  • RIV/00027006:_____/04:#####788!RIV06-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 515;519
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE-M01-01-03)
http://linked.open...iv/cisloPeriodika
  • 0
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 574352
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/04:#####788
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Kochia scoparia (L.) Schrad., herbicide resistance, acetolactate synthase inhibitors, genetic variation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [1CE1A5E526B0]
http://linked.open...i/riv/nazevZdroje
  • Zeitschrift für Pflanzenkrankheiten & Pflazenschutz
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 0
http://linked.open...iv/tvurceVysledku
  • Mikulka, Jan
  • Salava, Jaroslav
  • Chodová, Daniela
http://linked.open...n/vavai/riv/zamer
issn
  • 0340-8159
number of pages
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