About: Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population

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About: Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	PCR pro detekci granuloviru Cydia pomonella (CpGV) v larvách Cydia pomonella L. (obaleč jablečný) byla vyvinuta. Dále byly vyvinuty primery pro detekci transposonu TCp3.2 v larvách C. pomonella. (cs) The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection of CpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population. The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection of CpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population. (en)
Title	Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella (cs) Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population (en)
skos:prefLabel	Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella (cs) Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population (en)
skos:notation	RIV/00027006:_____/03:33!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	153;157
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE-M01-01-03)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Stará, Jitka Kocourek, František Kumar, Jiban
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	621522
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/03:33
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cydia pomonella; codling moth; Cydia pomonella granulovirus; PCR; transposon (en)
http://linked.open.../riv/klicoveSlovo	PCR Cydia pomonella granulovirus codling moth Cydia pomonella transposon
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[34B900369EC6]
http://linked.open...i/riv/nazevZdroje	Acta Virologica
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Kocourek, František Stará, Jitka Kumar, Jiban Pultar, O.
http://linked.open...n/vavai/riv/zamer	Výzkum ekologie, epidemiologie, šíření a metody diagnostiky škodlivých organismů, inovace systémů ochrany rostlin a skladovaných produktů
issn	0001-723X
number of pages	5 (xsd:int)

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