About: Rapid, specific detection of Listeria monocytogenes by antibody-based techniques     Goto   Sponge   Distinct   Permalink

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Description
  • The overall objective of the proposal is to develop rapid and specific immunoassay for the detection of Listeria in foods. To raise novel antibodies to peptide sequences representing Listeria surface proteins. Peptides will be bound on lysine tree. Antibodies obtained will be used in enzyme immunoassay (ELISA) for listeria detection. (en)
  • Cílem projektu je vyvinout rychlou a specifickou imunochemickou detekci listerií v potravinách Protilátky budou získány imunizací králíku peptidovými sekvencemi povrchových proteinu Listerií. Jejich imunogenicita bude zajištena navázáním peptidu na lysinový adukt. Získané protilátky budou vyuzity v systémy enzymové imunoanalýzy (ELISA) k detekci Listerií.
Title
  • Rapid, specific detection of Listeria monocytogenes by antibody-based techniques (en)
  • Rychlá specifická detekce Listerie monocytogenes pomocí protilátek
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  • OK 384
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  • Listeria, antibody, enzyme immunoassay, ELISA, foods (en)
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  • Byly vypracovány 2 imunochemické techniky, enzymová imunoanalýza a metoda %22dipstick%22 ke specifickémuprůkazu Listeria monocytogenes. Oba postupy využívají polyklonální protilátky proti povrchovému proteinu mikroorganismu - Internalinu B. (cs)
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  • Listeria
  • ELISA
  • antibody
  • enzyme immunoassay
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