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  • The project is focused on proteomic analysis of plant defense proteins and their protein-protein interactions. It concentrates on protease inhibitors that are biosynthesized in plants as insecticidal proteins. When ingested, they ively block the enzymatic activity of digestive proteases of insect herbivores. Plant protease inhibitors have potential application in plant biotechnologies and represent highly promising compounds for the development of new crops resistant to insect pests. The principal aim of the project is to design and apply proteomic tools for the identification and functional characterization of plant protease inhibitors and their target insect proteases. The project will increase our understanding of plant pathophysiology and complex molecular interactions between plants and insects. (en)
  • Cílem projektu je použít proteomické techniky k identifikaci obranných proteasových inhibitorů modelové rostliny a k charakterizaci jejich interakce s hmyzími trávicími proteasami.
Title
  • Functional proteomics of plant defense proteins (en)
  • Funkční proteomika obranných proteinů rostlin
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  • OC09007
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  • functional proteomics; plant defense proteins; protease inhibitors; plant-insect interaction (en)
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  • Projekt přinesl nové informace o mechanismech účastnících se interakce mezi obrannými proteiny rostlin a herbivorními hmyzími škůdci. Identifikovali jsme proteasové inhibitory exprimované v listech bramboru, které blokují funkci proteas v trávicím traktu mandelinky bramborové. Detailně jsme analyzovali interakci hlavní trávicí proteasy LdCD s inhibitorem PDI. (cs)
  • The project provides new information on the mechanism of interaction between plant defense proteins and herbivorous insect pests. We identified protease inhibitors expressed in potato leaves, which block the function of proteases in the digestive tract of Colorado potato beetle. The interaction of the major digestive protease LdCD with PDI inhibitor was analyzed in detail. (en)
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  • plant defense proteins
  • protease inhibitors
  • functional proteomics
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