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  • The objective of the proposed project is to continue the collaborative work initiated between , Palacky University in Olomouc, Dr. Ludmila Ohnoutkova (Czech Republic) and Spelman College, Dr. Mentewab Ayalew, (United States) towards the functional characterization of specific ATP Binding Cassette (ABC) transporters from barley (Hordeum vulgare L.). The aim of the work is to identify and functionally characterize barley ABC transporters that are orthologs of Atwbc19, an Arabidopsis ABC transporter that was shown to confer antibiotic resistance. Also will define a new ortholog genes. The presence and expression of transgene will be identified by molecular methods in the in vitro and in vivo condition. (en)
  • Projekt podporuje spolupráci mezi Univerzitou Palackého v Olomouci (Ludmilou Ohnoutkovou) a Spelman College (Mentewab Ayalew) USA, je zaměřen na funkční charakterizaci specifické ATP-vazebné kazet (ABC) transportérů ječmene (Hordeum vulgare L.). Účelem projektu je identifikace a funkční charakteristika ABC transportérů na pozadí jednoděložných rostlin homologních k Atwbc19 (member of the White-Brown Complex), který byl inentifikován u modelové rostliny Arabidopsis thaliana a vykazuje rezistenci ke kanamycinu. Definovány mohou být také nové ortologní geny. Exprese a funkce těchto genů bude ověřena u jednoděložných rostlin molekulárními metodami v in vitro a v in vivo podmínkách.
Title
  • Characterization of novel ABC transporters in monocots (en)
  • Nové geny ABC transportérů u jednoděložných rostlin
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  • LH13069
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  • ABC transportéry, aminoglykosidová antibiotika, ječmen, transformace (en)
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  • aminoglykosidová antibiotika
  • ječmen
  • ABC transportéry
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