About: Isotope-Coded Affinity Tag Protein Analysis     Goto   Sponge   NotDistinct   Permalink

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AttributesValues
rdf:type
rdfs:label
  • Isotope-Coded Affinity Tag Protein Analysis
rdfs:subClassOf
Semantic_Type
  • Research Activity
Preferred_Name
  • Isotope-Coded Affinity Tag Protein Analysis
UMLS_CUI
  • C1708579
Legacy_Concept_Name
  • Isotope-Coded_Affinity_Tag_Protein_Analysis
FULL_SYN
  • Isotope-Coded Affinity Tag Protein AnalysisPTNCI
  • ICAT AnalysisSYNCI
  • Isotope-Coded Affinity Tag AnalysisSYNCI
  • ICAT ProfilingSYNCI
  • ICATABNCI
DEFINITION
  • Recognizing that only a single tryptic peptide is needed to quantify the expression of the corresponding parent protein, the ICAT reagent was designed to affinity isolate and quantify via the use of a stable isotope the relative concentrations of cysteine-containing tryptic peptides obtained from digests of control versus experimental samples. A feature of the ICAT approach is that the in vitro incorporation of a stable isotope into one of the two samples being compared obviates the need to analyze by mass spectrometry the control and experimental samples separately. ICAT data, which is analogous to that obtained via the use of two different fluorescent dyes in DNA microarray analysis of mRNA or DIGE analysis of protein expression, provides the corresponding ratio for the level of expression of the parent protein in the control versus experimental sample.NCIhttp://keck.med.yale.edu/prochem/icat/
code
  • C54164
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