About: Molecular biology of fatty acid (amphiphilic) anion binding site of mitochondrial phosphate carrier     Goto   Sponge   NotDistinct   Permalink

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Description
  • Fatty acids(FA) are recently recognized to be key physiological regulators of oxidative phosphorylation (OxPho), causing partial uncoupling while being translocated via ADP/ATP car- rier as anions and returning protonated across the bilayer, thus resetting efficiency of OxPho. The process is undoubtedly important for cardiology, nutrition and all energetic related physiology. Recently, we have found that FA inhibit and bind to phosphate carrier (PiC), another key point of OxPho. We aim to locate FA binding site in the sequence of PiC by photoaffinity labeling with 3H-azido-FA , enzymatic cleavage and sequencing of resulted peptides and confirm it by site-directed mutagenesis of the given locus. Functionally, it will be investigated, whether FA interfere with a Pi binding site, OH- (or H+) translocation, whether affect HgCl2-induced uniport mode, if FA cycling mechanism is also valid for PiC, etc. (en)
  • Bodovými mutacemi a mapováním fotoafinně značených peptidů bude studováno vazební místo mastných kyselin (MK) u mitochondriálního fosfátového přenašeče a lokalizována jeho poloha v sekvenci. Transportní studie určí, zda MK kompetuje s fosfátem
Title
  • Molecular biology of fatty acid (amphiphilic) anion binding site of mitochondrial phosphate carrier (en)
  • Molekulární biologie vazebního místa mastných kyselin mitochondriálního fosfátového přenášeče
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  • ME 085
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  • Mitochondrial phosphate carrier, fatty acids, uncoupling, E-Coli expression, photoaffinity labelling (en)
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  • fatty acids
  • uncoupling
  • E-Coli expression
  • Mitochondrial phosphate carrier
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